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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2005-11 to 2006-03
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions: analytical method no described in enough detail

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 B (Bioaccumulation: Semi-static Fish Test)
Version / remarks:
adopted May 1981
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
4,4-Difluoro Benzophenone
IUPAC Name:
4,4-Difluoro Benzophenone
Details on test material:
Physical state: white powder
Purity: 99%
Radiolabelling:
no

Sampling and analysis

Details on sampling:
- Sampling intervals/frequency for test organisms: at 30 h, 42 h, 72 h, 96 h, 120 h, 144 h, 240 h, 360 h
- Sampling intervals/frequency for test medium samples: at 0 h, 24 h, 48 h, 72 h, 96 h, 120 h, 144 h, 288 h
- Sample storage conditions before analysis: frozen
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods):

Test solutions

Details on preparation of test solutions, spiked fish food or sediment:
A stock solution with a concentration of 100 mg/mL was prepared in acetone

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- fish were acclimated to laboratory conditons for 2 weeks
- length: 25 - 35 mm, weight: 0.2 - 0.4 g

Study design

Route of exposure:
aqueous
Test type:
semi-static
Water / sediment media type:
not specified
Remarks:
freshwater
Total exposure / uptake duration:
15 d
Total depuration duration:
8 d

Test conditions

Test temperature:
24 +/- 2 °C
pH:
6.95 to 7.04
Dissolved oxygen:
7.9 to 8.1 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- 120 L glass aquaria, filled with 100 L test solution
- Aeration: no:
- Renewal rate of test solution: every 24 hours
- No. of organisms per vessel: 150
- No. of vessels per concentration (replicates): 1
Nominal and measured concentrations:
Nominal: 0.1 mg/L and 0.2 mg/L
Details on estimation of bioconcentration:
- no data

Results and discussion

Bioaccumulation factoropen allclose all
Type:
BCF
Value:
55.4
Basis:
whole body w.w.
Time of plateau:
240 h
Calculation basis:
steady state
Remarks on result:
other: Conc.in environment / dose:0.1 mg/L
Type:
BCF
Value:
74.4
Basis:
whole body w.w.
Time of plateau:
240 h
Calculation basis:
steady state
Remarks on result:
other: Conc.in environment / dose:0,2 mg/L
Depuration
Elimination:
yes

Any other information on results incl. tables

Concentration (mg/L)

Cf
(mg/kg, mean value)

Cw

(mg/L, mean value)

BCF

0.1

5.045

0.091

55.4

0.2

12.581

0.169

74.4

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Executive summary:

The bioaccumulation of 4,4-Difluoro Benzophenone was investigated in a bioaccumulation study with fish according to OECD 305 B. The test species Danio rerio was exposed to the test item at two concentration levels (0.1 and 0.2 mg/L) The uptake phase lasted 15 days (until the steady state was reached) and subsequent elimination was monitored over a period of 8 days. The following steady state BCF values were calculated

Conc. A (0.1 mg/L): BCF = 55.4

Conc. B (0.2 mg/L): BCF = 74.4

The study was assessed as "reliable with restrictions".

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