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Luperox 610 is not a peroxisome inducer in rat hepatocyte primary cultures.

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The possible peroxisome proliferation in vitro induced by Luperox 610 was evaluated in rat hepatocyte primary cultures following 4 days of treatment (Dechariaux, 1994). The method used is that described by Lazarow (1981) which consists in measuring the increase in palmitoyl-CoA-oxidase activity in rat hepatocyte primary cultures treated at various concen­trations. Enzyme assays were performed by a radio-chemical method. The first peroxisome proliferation assay (HPC73) was performed using a maximal concentration of 200 µg/ml. This concentration was selected according to the solubility of the compound in the culture medium. In view of the high toxicity of the substance, a second study (IIPC73A) was performed at a top concentration of 37.5 µg/ml. After the 96-hour culture period, the test substance was highly toxic from 50 µg/ml in the first study; the toxic effect was less pronounced at 37.5 µg/ml in the second study. No toxic effect was noted at 25 µg/ml (first study) or at 1, 5, 10 and 25 µg/ml (second study). The test substance did not induce any increase in palmitoyl-CoA-oxidase activity at 25 µg/ml (first study) or at 1, 5, 10 and 25 µg/ml (second study). In conclusion, Luperox 610 is not a peroxisome inducer in rat hepatocyte primary cultures in vitro following daily treatment for 4 days at concentrations of 1, 5, 10 and 25 µg/ml.