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Ecotoxicological information

Toxicity to soil microorganisms

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Description of key information

The effect of the test item on the nitrogen transformation activity of the soil microorganisms gave an EC50 of greater than 1000 mg/kg. Correspondingly the No Observed Effect Concentration (NOEC) was equal to or greater than 1000 mg/kg.

Key value for chemical safety assessment

Short-term EC50 for soil microorganisms:
1 000 mg/kg soil dw

Additional information

A study was performed to assess the long-term effect of the test item, after a single exposure, on nitrogen transformation activity of soil microorganisms. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2000) No 216 "Soil Microorganisms: Nitrogen Transformation Test" referenced as Method C.21 of Commission Regulation (EC) No. 440/2008; Study performed in accordance with the Principles of Good Laboratory Practice (GLP) and reported with a valid GLP certificate.

 

Methods

Following a preliminary range-finding test soil microorganisms were exposed to the test item at a single concentration of 1000 mg/kg for 28 days at a temperature of approximately 21 °C, in the dark with the addition of powdered Lucerne-green-grass meal to act as a respiratory substrate.

 

The inhibitory effect of the test item on nitrogen transformation was assessed by the determination of nitrate concentration in the soil samples on Days 0, 7 and 28 and compared to data obtained from control soil samples.

 

Results

The effect of the test item on the nitrogen transformation activity of the soil microorganisms gave an EC5oof greater than 1000 mg/kg. Correspondingly the No Observed Effect Concentration (NOEC) was equal to or greater than 1000 mg/kg.