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EC number: 418-310-3 | CAS number: 126050-54-2
The effect of ADK STAB HP-10 on the respiration rate of activated sludge was assessed by the methods detailed in EC Directive 88/302, 'Biodegradation - Activated Sludge Respiration Inhibition test' and OECD Test Guideline 209, 'Activated Sludge, Respiration Inhibition test'.
Samples of activated sludge (suspended solids 1.6 g/l), fed with synthetic sewage, were exposed to the test substance at nominal concentrations of 1, 10 and 100 mg/l for three hours. Single mixtures were prepared at 1 and 10 mg/l and the highest level was prepared in triplicate. Their rates of oxygen consumption were determined and compared with those of controls, containing activated sludge and synthetic sewage alone, which were established at the beginning and end of the culture series.
The reference inhibitor 3,5-dichlorophenol (3,5-DCP) was employed at 3.0, 10.0 and 32.0 mg/l, as a positive control.
The specific respiration rate of the control culture established at the end of the test series
(38.5 mgO2/g/h) was 90% of the rate of that established at the start (42.9 mgO2/g/h). The three-hour 50% effect concentration (EC50) for 3,5 -DCP was calculated to be 10.4 mg/1 (95% confidence limits 7.1 - 15.5 mg/l). These results show that the test was valid and that the sample of activated sludge employed was sensitive to inhibition.
ADK STAB HP-10 had no inhibitory effect on the respiration rate of activated sludge at any of the concentrations employed in the test. The EC20, EC50 and EC80 of the test substance could not, therefore, be calculated but these must be greater than 100 mg/l, the highest level tested.
Key value determined using EU Method C11 and OECD guideline 209.
The effect of ADK STAB HP-10 on the respiration rate of activated sludge was assessed.
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