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EC number: 418-310-3 | CAS number: 126050-54-2
Table 1: Incidence of immobility in the range-finding test:
Concentration Test substance (mg/l)
Number Daphnia exposed
Response at 24h
Response at 48h
*Thin parts of substance were observed at the surface of the test solution.
Table 2: Acute immobilization of daphnia after 24 and 48 hours in the first EC50-test with various concentrations of ADK STAB HP-10
Concentration substance (mg/l)
Number immobile at 24h
Number immobile at 48h
1.9 - 0.2
*Thin scales of test substance were observed at the surface of the solution.
n.m. = Not measured by analysis of samples.
Table 3: Acute immobilization of daphnia after 24 and 48 hours in the second EC50-test with various concentrations of ADK STAB HP-10
1Initial concentrations measured in samples taken at the start of the exposure.
*Thin scales of the test substance were observed at the surface of the solution.
n.d. = not detected.
Acute Toxicity study in Daphnia magna with ADK STAB HP-10.
The study procedures described in this report were based on the EEC directive 92/69, Part C.2. "Acute toxicity for Daphnia" and the OECD guideline No. 202: "Daphnia sp., Acute Immobilisation Test", Adopted April 4, 1984,
The water solubility of ADK STAB HP-10 in distilled water at 20.0±0.5°C is determined to be< 0.59 mg/l, using the column elution method (NOTOX Project 155442). The partition coefficiant (n-octanol/water) of ADK STAB HP-10 was determined to be>1.6 x 106(log Pow>6.2) at room temperature (NOTOX Project 155453).
Daphnia were exposed for a maximum of 48 hours in two separate tests to a range of nominal concentrations from 18 to 180 mg/l and 0.45 to 10 mg/l, respectively. The test solutions in the first test were prepared starting with stocks in tetra-hydrofuran (THF) and those in the second test were prepared with stocks in acetone. Both tests were performed in duplicate with 10 daphnia per vessel.
All concentrations of ADK STAB HP-10 tested exceeded its solubility in water. The 48h-EC50 depended on the way the test solutions were prepared. The sensitivity of the different batches of daphnia used were considered to be similar as the 48-hour EC50 was 0.6mg/l in the corresponding reference tests with potassium dichromate.
When test medium was exposed to ADK STAB HP-10 concentrations present in test vessels after evaporation of the so1vent THF, the nominal 48h-EC50 corresponded with 51 mg/l (95% confidence interval between 39 and 74 mg/l). However, the analysis of samples did not provide reliable information about the corresponding actual exposure concentrations. Further, the effect on mobility at the higher nominal concentrations was probably related to the presence of undissolved test substance particles.
When test medium was mixed with volumes of ADK STAB HP-10 stocks in acetone, the 48h-EC50 approximated a nominal concentration of 10 mg/l. Nominal concentrations of 4.5 mg/l and lower did not induce acute immobilization of Daphnia magna after 48 hours of exposure (NOEC). The actual exposure concentrations decreased substantially during the 48-hour test period owing to the fact that this highly hydrophobic test substance precipitated out of the water phase.
In conclusion, these results showed that ADK STAB HP-10 was not toxic for Daphnia magna at levels beyond its water solubility (< 0.59 mg/l), except in those cases where initial concentrations measured exceeded 2.4 mg/l, and the fraction of undissolved test substahce particles reached amounts that most likely induced physical damage to Daphnia magna.
Key value determined using EU Method C2 and OECD guideline 202.
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