C-C1

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

31st March 2007 to 1st May 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of relevant results

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

yes

Remarks:

Substance tested in ionised form

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

yes

Remarks:

Substance tested in ionised form

GLP compliance:

yes (incl. QA statement)

Remarks:

Date of inspection: 30th August 2005 Date of signature: 21st November 2005

Type of method:

HPLC estimation method

Media:

soil

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

Sponsor's identification :C-C1
Description : dark blue powder
Lot number : MB-1
Date received : 05 March 2007
Storage conditions :room temperature in the dark, over silica gel

Radiolabelling:

no

Study design

Test temperature:

30°C.

HPLC method

Details on study design: HPLC method:

MOBILE PHASES
- Experiments with additives carried out on separate columns: yes
- pH: 6.35 to 7.11
- Solutes for dissolving test and reference substances: Test material (0.0100 g) was diluted to 100 ml with reverse osmosis water.

DETERMINATION OF DEAD TIME
- Method: by means of homologous series

REFERENCE SUBSTANCES
Acetanilide
Phenol
Atrazine
Isoproturon
Triadimenol
Linuron
Endosulfan-diol
-Endosulfan
Phenanthrene
DDT


DETERMINATION OF RETENTION TIMES
- HPLC System : Agilent Technologies 1050, incorporating autosampler and workstation
Column : Zorbax 5B-CN 5µ (250 x 4.6 mm id)
Column temperature :30ºC
Mobile phase : methanol:reverse osmosis water (55:45 v/v)
pH of mobile phase :6.35 to 7.11
Flow-rate : 1 ml/min
UV detector wavelength :dead time and reference standards: 210 nm
sample: 330 nm
Injection volume :10 µl


REPETITIONS
- Number of determinations:

EVALUATION
- The dead time was determined by measuring the retention time of formamide (purity* 99.5%, 1.25 x 103 mg/l solution in methanol:reverse osmosis water (55:45 v/v).

A calibration curve was constructed from the retention time data of the formamide and reference standard solutions. The capacity factors (k) for the reference standards were calculated.

log10 Koc value determined with reference to the calibration curve

Results and discussion

Adsorption coefficient

Type:

log Koc

Value:

< 1.25

Results: HPLC method

Details on results (HPLC method):

- Retention times of reference substances used for calibration: Please see section "Any other information on results)
- Details of fitted regression line (log k' vs. log Koc):
- Graph of regression line attached Yes
- Average retention data for test substance:

Any other information on results incl. tables

The retention times of formamide and the retention times, capacity factors (k) and log₁₀K_ocvalues for the reference standards are shown in the two following tables:

Dead Time	Retention Time (mins)		Mean Retention Time (mins)
	Injection 1	Injection 2
Formamide	2.881	2.878	2.880

Standard	Retention Time (mins)		Mean Retention Time (mins)	Capacity Factor (k)	Log10k	Log10Koc
	Injection 1	Injection 2
Acetanilide	4.125	4.128	4.127	0.433	-0.363	1.25
Phenol	3.930	3.935	3.933	0.366	-0.437	1.32
Atrazine	6.169	6.171	6.170	1.143	5.79 x 10^-2	1.81
Isoproturon	6.644	6.652	6.648	1.309	0.117	1.86
Triadimenol	9.342	9.351	9.347	2.246	0.351	2.40
Linuron	16.775	16.799	16.787	4.830	0.684	2.59
Endosulfan-diol	12.388	12.411	12.400	3.306	0.519	3.02
a-Endosulfan	23.714	23.535	23.625	7.204	0.858	4.09
Phenanthrene	18.461	18.447	18.454	5.409	0.733	4.09
DDT	57.283	57.271	57.277	18.891	1.276	5.63

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Remarks:

See comments in 'Overall remarks, attachments'.

Conclusions:

The adsorption coefficient (Koc) of the test material has been determined to be <17.8, log10 Koc <1.25.

Executive summary:

The determination was carried out using the HPLC screening method, Method C19 of Commission Directive 2001/59/EC (which constitutes Annex V of Council Directive 67/548/EEC) and Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001.

The adsorption coefficient (K_oc) of the test material has been determined to be <17.8, log₁₀ K_oc <1.25.