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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

EC50 (48 h) > 2.2 mg/L, which constitutes the limit of solubility of the test item under the test conditions.

Key value for chemical safety assessment

Additional information

Isononyl benzoate was tested in acute toxicity test with daphnids (Daphnia magna Straus) under static conditions according to the guideline EU Method C.2 and OECD Test Guideline 202 (Acute Toxicity for Daphnia). The test was conducted as limit test. In order to attain the solubility limit of the test substance, which is poorly soluble in water, 1 g test substance /L was stirred for 18 h and undissolved test substance was then removed by filtration. The measured test concentration after 48 h deviated by more than 20% from the freshly prepared test solution. Therefore, the biological value was calculatet with the geometrical mean of the analytical concentration after 0 and 48 hours. No effects on the exposed daphnids were recorded, thus the EC50 is > 2.2 mg/L which constitutes the solubility limit of Isononyl benzoate under the conditions of the test.