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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: OECD Guideline study

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes (incl. QA statement)

Test material

Details on test material:
Chemical Name:3-[(2,2-Dimethyl-1 ,2-azasilolidin-1-yl)(dimethyl)silyl]-l-propanamin
Aggregate State: liquid
Storage: refrigerator
Stability: instable in water (::=; 1.0 min.)
Safety Precautions: Routine hygienic procedures were sufficient to assure personnel health and safety

Study design

Oxygen conditions:
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
supplied by the sewage plant Groß-Simmern, Germany
The activated sludge used for this study was washed by centrifugation and the supematant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 1.5 g dry material per litre were mixed with test water and then aerated until use.
Duration of test (contact time):
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
The amounts of test item and reference item were directly weighed into the test flasks. No emulsifiers or solvents were used, but the solutions were dispersed by stirring to achieve a homogeneous solution of the test item.
The closed test flasks were incubated in a climatic chamber under continuous stirring. The consumption of oxygen was determined by measuring the change of pressure in the flasks.
Evolved carbon dioxide was absorbed in an aqueous solution (45 %) of potassium hydroxide.
Temperature was measured each working day in the climatic chamber.
pH-values were measured in all flasks at the start and end of the test using a pH-electrode WTW pH 340i
Surrounding Type: climatic chamber
Temperature: 21 - 22°C
Light Conditions: darkness
pH-Value of Test Solutions: 7.6 (measured at the start of the test)
Manometric Test System with test flasks containing a volume of 500 mL
Reference substance
Reference substance:

Results and discussion

% Degradation
% degradation (O2 consumption)
St. dev.:
Sampling time:
28 d
Details on results:
Inoculum Control: The oxygen demand of the inoculum control (medium and inoculum) was 25 and 30 mg 02/L and was thus not greater than 60 mg O2/L within 28 days.
pH-Value: The pH-value of the test item flasks at the end of the test was 7.8 and thus within the range of pH 6.0 - 8.5.
Reference Item: The percentage degradation of the reference item Aniline reached the level for ready biode-gradability (> 60 %, based on ThODNH4) within 4 days.
Test Item: The difference of duplicate values for the degradation of the test item at any time during the test was less than 20 %.

BOD5 / COD results

Results with reference substance:

0 % degradation after 1 d
63 % degradation after 4 d
81 % degradation after 7 d
99 % degradation after 14 d
100 % degradation after 21 d

Applicant's summary and conclusion

Validity criteria fulfilled:
Interpretation of results:
under test conditions no biodegradation observed
The test item can be considered to be not readily biodegradable.
Executive summary:

The test item was investigated for its ready biodegradability in a Manometric Respirometry Test (OECD 301 F) over a period of 28 days. The biodegradation was followed by the oxygen uptake of the micro organisms during exposure. As a reference item Aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. After correction for the mean biochemical oxygen demand of the inoculum controls at the end of the 28-day exposure period degradation rates of -1%and 1%,based on ThODNH4and ThODN03,were found. The test item can therefore be considered to be not readily biodegradable. The reference item Aniline was sufficiently degraded to 99%after 14 days, and to 109 % after 28 days of incubation, based on ThODNH4,thus confirming the suitability of the used activated sludge inoculum. In the toxicity control containing both, the test item and the reference item Aniline, 41 % biodegradation was noted within 14 days and 44%biodegradation was determined after 28 days of incubation, based on ThODNH4 •Thus, the test item can be assumed to be not inhibitory on the activated sludge micro organisms.