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Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
05 May - 15 October 2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes
Type of method:
HPLC estimation method
Media:
soil/sewage sludge
Radiolabelling:
no
Test temperature:
35°C +/- 1.0°C
Details on study design: HPLC method:
EQUIPMENT
Instrument: Alliance Separation Module 2695
Detector: Dual λ Absorbance Detector 2487 (Waters)
Column: 150 mm x 4.6 mm i.d. Hypersil BDS-CN; dp = 5 µm (Thermo, San Jose, CA, USA)
Column temperature: 35°C +/- 1.0°C
Mobile phase: 55/45 (v/v) methanol/water
Flow : 1.0 ml/min
Injection volume: 10 µl
UV detection: 210 nm


REFERENCE SUBSTANCES
- Identity: A stock solution of phenol in methanol at a concentration of 1.23 g/l was used. The phenol reference substance solution was prepared by adding 50 µl of the stock solution to a 5 ml volumetric flask. The flask was filled up to the mark with mobile phase.


TEST SOLUTIONS:
A 884 mg/l stock solution of the test substance was prepared in methanol. In order to dissolve the test substance completely the stock solution was ultrasonicated for 10 minutes.
The test solution was prepared by adding 50 µl of the stock solution to a 5 ml volumetric flask. The flask was filled up to the mark with mobile phase. The final concentration of the test substance solution was 8.84 mg/l.


REPETITIONS
- Number of determinations: The reference substance and test substance solutions were injected in duplicate. Blank solutions were analysed by single injection.
Analytical monitoring:
no
Details on matrix:
The dual composition of a cyanopropyl stationary phase, having polar and non-polar sites allows for interaction of polar and non-polar groups of a molecule in a similar way as is the case for organic matter in soil or sewage sludge matrices. This enables the relationship between the retention time on the column and the K,0 on organic matter to be established.
Details on test conditions:
TEST CONDITIONS
- pH: neutral
Key result
Sample No.:
#1
Type:
Koc
Value:
< 20.9 dimensionless
pH:
7
Temp.:
35 °C
Matrix:
The final concentration of the test substance solution was 8.84 mg/I.
Key result
Sample No.:
#1
Type:
log Koc
Value:
< 1.32 dimensionless
pH:
7
Temp.:
35 °C
Matrix:
The final concentration of the test substance solution was 8.84 mg/I.
Details on results (HPLC method):
- Retention times of reference substances used for calibration: retention time of phenol (log Koc = 1.32) was 2.30 minutes

- Average retention data for test substance: one peak with a retention time of 1.97 minutes was observed
Transformation products:
not measured
Validity criteria fulfilled:
yes
Conclusions:
The Koc value of the test substance is: < 20.9
The log Koc value of the test substance is: < 1.32
All measurements were performed in neutral pH.

Description of key information

The Koc value of the test substance is: < 20.9

The log Koc value of the test substance is: < 1.32

All measurements were performed in neutral pH.

Key value for chemical safety assessment

Additional information