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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
5 - 9 July 1999
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Test method used is according to method 71 of Japanese Industrial Standards (JIS) K0102. The study is considered to be reliable with restrictions since the recoveries of the analytical method were not reported and the final dispersant (HCO-40) concentrations were above the maximum mentioned on OECD 203 guideline.
Reason / purpose for cross-reference:
reference to same study
Qualifier:
equivalent or similar to guideline
Guideline:
other: Test method according to method 71 of Japanese Industrial Standards (JIS) K0102. This test method is essentially the same as that in the OECD Guidelines for Testing of Chemicals "Bioconcentration : Flow-Through Fish Test; 305C, Modified MITI Test".
Deviations:
yes
Remarks:
- as compared to OECD guideline 203 (1992)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: blank-control, solvent control, 10, 18, 32, 56, 100 mg/L
- Sampling: About 5 mL of the test solution was sampled at 0, 24, 72 and 96 h.
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 1 g test substance and 20 g HCO-40 were weighed and dissolved in acetone. Acetone was evaporated at 40°C. The residue was dissolved in 200 mL of pure water as the stock solution (5000 mg/L). The test solutions were prepared from this stock.
The solvent control was prepared by dissolving 6 g of HCO-40 in acetone. Acetone was evaporated at 40°C. The residue was dissolved in 100 mL of pure water as the solvent stock solution (60000 mg HCO-40/L). This solution was added to dilution water to obtain the desired solvent concentration.
- Controls: blank-control and solvent control
- Chemical name of vehicle (emulsifier/dispersant): HCO-40
- Concentration of vehicle in stock solutions:
Used to prepare the test concentrations: 100 g/L
Used to prepare the solvent control: 60 g/L
- Concentration of vehicle in test medium
Final test solution (highest test concentration): 2 g/L
Solvent control: 2 g/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): Not reported
Test organisms (species):
Oryzias latipes
Details on test organisms:
TEST ORGANISM
- Common name: Ricefish
- Source: Uemura Fish Farm, 32-1, Sugi-cho, Yamatokoriyarna, Nara, Japan. Lot No. M99D-01
- Length at study initiation (mean and SD): 2.6 ± 0.1 cm
- Weight at study initiation (mean and SD): 0.17±0.02 g
- Feeding during test: No

ACCLIMATION
- Acclimation period: The acclimation begun April 5, 1999 in a flow-through tank without sterilization and disinfection. The water temperature of the acclimation tank was kept at 25 ± 2 °C.
- Acclimation conditions (same as test or not): yes
- Type of food: Fodder (not further specified)
- Feeding frequency: Fish (ricefish) were fed every day (once or twice). Occasionally feeding was done every two days (also once or twice). The feeding was stopped two days before the start of the exposure period.
- Health during acclimation (any mortality observed): During the 7 days prior to the start of the exposure period, 2.0% of all fish in the acclimation tank were removed because they were weakened or dead.
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
Not reported
Test temperature:
24.6-25.0 °C
pH:
7.6-8.2
Dissolved oxygen:
6.8-8.1 mg/L
Nominal and measured concentrations:
- Nominal: blank-control, solvent control, 10, 18, 32, 56, 100 mg/L

- Measured:
0 h: 0.0 (blank-control), 0.0 (solvent-control), 10.1 (10), 18.4 (18), 32.8 (32), 58.8 (56) and 97.0 (100) mg/L
24 h (old test solution): 0.0 (blank-control), 0.0 (solvent-control), 10.2 (10), 18.4 (18), 32.0 (32), 56.4 (56) and 98.0 (100) mg/L
72 h (freshly prepared test solution): 0.0 (blank-control), 0.0 (solvent-control), 10.1 (10), 17.7 (18), 32.2 (32), 54.4 (56) and 100 (100) mg/L
96 h (old test solution): 0.0 (blank-control), 0.0 (solvent-control), 9.9 (10), 18.3 (18), 31.6 (32), 55.6 (56) and 96.0 (100) mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 3 L of capacity, filled with 3 L of test solution
- Aeration: Not reported
- Renewal rate of test solution (frequency): Test solutions were renewed once at 72 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.6 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated city water (fully aerated)
- Chlorine: Residual chlorine concentration was below 0.02 mg/L
- Culture medium different from test medium: No
- Intervals of water quality measurement: Not reported

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Not reported

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortality and abnormalities. The fish were observed at 24, 48, 72 and 96 hours of exposure.
Reference substance (positive control):
yes
Remarks:
Pentachlorophenol sodium salt (PCP)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Measured concentrations: The concentrations of the test substance in the test solutions were measured at initiation, 24, 72 and 96 h. All measurement values were within ± 20% of nominal concentrations.

- Biological results: No mortality or abnormalities were observed in the blank and solvent controls and in all test concentrations. Only at 18 mg/L test concentration 100% mortality occurred after 48-h, but due to a fault.

Acceptability of the test (according to OECD guideline 203, 1992):
1. No mortality was observed in the control at the end of the test.
2. Test conditions were maintained constant throughout the test.
3. The dissolved oxygen concentration has been at least 60% of the air saturation value throughout the test.
4. The concentrations of the substance being tested have been at least 80% of the nominal concentration throughout the test.
Results with reference substance (positive control):
PCP: 96 h LC50 was 0.40 mg/L
Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test SPS-100 induced no visible or lethal effects in ricefish at an analytically confirmed nominal concentration of 100 mg/L. Threfore, the 96h LC50 was concluded to exceed 100 mg/L.
Executive summary:

In a 96-h acute toxicity study conducted according to method 71 of JIS K0102, ricefish (Oryzias Iatipes) were exposed to SPS-100 under semi-static conditions at the following nominal concentrations: blank-control, solvent control, 10, 18, 32, 56, 100 mg/L. Test substance induced no visible or lethal effects in ricefish atany of the concentrations tested.The 96 h LC50 was >100 mg/L based on analytically confirmed nominal concentrations. The study is considered to be reliable with restrictions since the recoveries of the analytical method were not reported and the final dispersant (HCO-40) concentrations were above the maximum mentioned on OECD Guideline No. 203.

Description of key information

In a 96-h acute toxicity study conducted according to method 71 of JIS K0102, ricefish (Oryzias Iatipes) were exposed to SPS-100 under semi-static conditions. The 96-h LC50 was >100 mg/L based on analytically confirmed nominal concentrations. The study is considered to be reliable with restrictions since the recoveries of the analytical method were not reported and the final dispersant (HCO-40) concentrations were above the maximum mentioned on OECD Guideline No. 203.

Key value for chemical safety assessment

Additional information

In a 96-h acute toxicity study conducted according to method 71 of JIS K0102, ricefish (Oryzias Iatipes) were exposed to SPS-100 under semi-static conditions at the following nominal concentrations: blank-control, solvent control, 10, 18, 32, 56, 100 mg/L. Test substance induced no visible or lethal effects in ricefish atany of the concentrations tested.The 96 h LC50 was >100 mg/L based on analytically confirmed nominal concentrations. The study is considered to be reliable with restrictions since the recoveries of the analytical method were not reported and the final dispersant (HCO-40) concentrations were above the maximum mentioned on OECD Guideline No. 203.