Bis(2-ethylhexyl) 2-(4-hydroxy-3,5-dimethoxybenzylidene)malonate

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study performed according to OECD 207 under China GLP regulation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

GLP compliance:

yes

Remarks:

China GLP regulation

Test material

Reference

Name:

Unnamed

Type:

Constituent

Test material form:

liquid: viscous

Details on test material:

Chemical name: 2-(4-Hydroxy-3,5-dimethoxy-benzylidene)-malonic acid bis-(2-ethyl-hexyl) ester
CAS No.: 444811-29-4

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

The test item was directly added into the soil. A total amount of 10 g mixture of the test item and dry soil was prepared (premix). Then the premix was added to 540 g soil (dry weight) for each test container. The medium was throughly mixed and deionised water was added to give an overall moisture content of about 35 %.

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

The test organisms were obtained from a eartworm farm in Nanjing, China

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test conditions

Test temperature:

20 - 22°C

pH:

6.2 - 6.5

Moisture:

30 - 36 %

Nominal and measured concentrations:

1000 mg/kg (dry weight of soil)

Reference substance (positive control):

yes

Remarks:

2-chloracetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil ww

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/kg soil ww

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Details on results:

No mortality were observed in worms exposed to the test item at a limit concentration of 1000 mg/kg soil ww during the 14-day exposure period.

Results with reference substance (positive control):

The LC50 0f 2-chloroacetamide to Eisenia fetida was 25.1 mg/kg (dry soil) after 14 days of exposure.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test item did not induce any toxicity to earthworms exposed to the limit concentration of 1000 mg/kg soil over a period of 14 days.

Executive summary:

The acute toxicity of the substance to adult earth worm (Eisenia fetida) was investigated in an artificial soil test according to OECD No. 207. Artificial soil was used as the test medium. The moisture content of the test medium during the course of the study was between 30 and 36% and the pH was between 6.2 and 6.5. The earth worms were exposed to a limit dose of 1000 mg/kg dry weight soil for a period of 14 days at defined temperature (20 - 22°C), humidity (80 to 85%) and under continuous light (400 to 800 lux). Each group (control and treatment group) consisted of 4 replicates with 10 worms each. No lethality was observed during the 14 -day observation period neither in the control nor in the limit treatment group. Based on the results of this study, the LC50 of the test substance exceeded 1000 mg/kg dry soil. This concentration can be considered as the NOEC as no adverse effects have been observed in the treatment group.