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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008
Reliability:
1 (reliable without restriction)
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
yes
Remarks:
Range-finding and final test were performed in Petri-dishes to reduce the light path by reducing the depth of the coloured solutions. Variation on section by section growth rate in the control group was above 35% at end of the range finding test (i.e. 45%
GLP compliance:
yes (incl. QA statement)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
0.32, 1.0, 3.2, 10, 32 and 100% of a Water Soluble Fraction (WSF) prepared at a loading rate of 100 mg/l.

Test solutions

Vehicle:
no
Details on test solutions:
Preparation of test solution started with a loading rate of 100 mg/l applying a 15-minute treatment period with ultrasonic waves followed by 35 minutes of magnetic stirring to obain maximum solubility in the test medium. Subsequently, the mixture was left to stabilise for 2 hours after which the Water Soluble Fraction (WSF) was siphoned off. However, very small particles were observed in this WSF. Therefore, the WSF was left to stabilise for another 30 minutes and a second WSF was siphoned off. The lower test concentrations were prepared by subsequent dilutions of the second WSF in test medium. The final test solution were all clear and ranged from colourless to dark pink.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Strain: NIVA CHL 1

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
no

Test conditions

Hardness:
n.a.
Test temperature:
21.9- 23.1°C
pH:
8.2-8.3 at the start to 8.0-8.1 at the end of the test
Dissolved oxygen:
n.a.
Salinity:
n.a.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
12 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 7.7-18
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
1.2 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 0.39-3.7
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
1.1 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 0.69-1.7
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.19 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 0.06-0.64
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.21 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
< 0.21 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
biomass
Details on results:
Note that the concentrations, in which effects were observed, were all coloured. Colour ranged from very slightly pink at the 1.0% WSF to dark pink at the 100% WSF. It can therefore not be excluded that at least part of the effect was due to absorption of wavelengths necessary for algal growth by the colour of the test solution.
Results with reference substance (positive control):
- Results with reference substance valid? yes

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 for growth rate reduction was 12 mg/l.