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EC number: -
CAS number: -
Percentage Biodegradation Values :
% Degradation Sodium Benzoate Procedure Control
% Degradation Test Item
% Degradation Test Item plus Sodium Benzoate Toxicity Control
pH Values of the Test Preparations on Days 0 and 28 :
prior to adjustment on Day 0
after adjustment on Day 0
pH on Day28
Inoculum Control R1
lnoculum Control R2
Procedure Control R1
Procedure Control R2
Test Item R1
Test Item R2
Total and Inorganic Carbon Values in the Culture Vessels on Day 0 :
IC/TC Ratio (%)
Test Item 10mgC/L R1
10 mg C/L R2
R1 - R2 = Replicate 1 and 2
* Corrected for control values.
** Total carbon value given is the sum of the TC value obtained from analysis and the nominal TC contribution of the test item
An experimental study was performed to assess the ready biodegradability of the test item in an aerobic aqueous medium according the OECD Guidelines for Testing of Chemicals (1992) No. 301B, "Ready Biodegradability; CO2 Evolution Test" and GLP. The test item, at a concentration of 10 mg Carbon/L, was exposed to activated sewage sludge micro-organisms with mineral medium in sealed culture vessels in the dark at approximately 21 °C for 28 days. The degradation of the test item was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the reference item, sodium benzoate, together with a toxicity control were used for validation purposes.
The test item attained 26% degradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301B.
An experimental study was performed to assess the ready biodegradability of the test item in an aerobic aqueous medium according the OECD Guidelines No. 301B and GLP. The test material attained 26% degradation after 28 days therefore is not considered to be readily biodegradable.
The REACH guidance document R7B indicates that biodegradation above 20% may be regarded as evidence of inherent biodegradation (for more details, please see the REACH gidance document R7b v4, June 2017, page 216)
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