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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
adopted July 17, 1992
GLP compliance:

Test material

Constituent 1
Reference substance name:
methyl tetradecanoate, chloro
Molecular formula:
C13H26Cl4COOCH3 and C13H25Cl5COOCH3 (primary isomers based on product weight of 35-40% Cl by weight)
methyl tetradecanoate, chloro
Constituent 2
Reference substance name:
Methyl octadecanoate, chloro
Molecular formula:
C17H30Cl4COOCH3, C17H29Cl5COOCH3 , and C17H28Cl6COOCH3 (primary isomers based on 35-40% Cl by weight)
Methyl octadecanoate, chloro
Constituent 3
Reference substance name:
Methyl hexadecanoate, chloro
Molecular formula:
C15H28Cl4COOCH3 and C15H27Cl5COOCH3 (primary isomers based on 35-40% Cl by weight)
Methyl hexadecanoate, chloro
Test material form:
Details on test material:
This is a UVCB substance developed from the chlorination of fatty acid methyl esters.
Specific details on test material used for the study:
Batch #A070806

Sampling and analysis

Analytical monitoring:

Test solutions

Details on test solutions:
According to the results of a pre-experiment, DA 8506 is not soluble at a concentration of 100 mg/L in the test water. Therefore, three WAFs (water accommodated fractions) with loading rates of the test item of 1.0, 10 and 100 mg/L were prepared. Two individual dispersions of the test item with loading rates of 10 and 100 mg/L were prepared by weighing adequate amounts of the test item by means of an analytical balance:
Loading rate 10 mg/L: 50.1 mg suspended in 5000 ml of test water
Loading rate 100 mg/L: 301.8 mg suspended in 3000 ml of test water

The test item was dispersed in the test media using ultrasonic treatment for 15 minutes. Then, the dispersions were stirred by magnetic stirrers at room temperature in the dark over 96 hours to dissolve a maximum amount of the test item in the test media. No auxiliary solvent or emulsifier was used. After the stirring period of 96 hours, each of the dispersions was filtered through a membrane filter (Schleicher & Schuell, Type NC45, pore size 0.45 μm). The filtrates were tested on the fish as WAFs.

The WAF of the lowest loading rate of 1.0 mg/L was prepared by dilution (1: 10) of the WAF with the loading rate of 10 mg/L with test water, due to technical reasons.

The finished test solutions were clear with no visible test material.

Additionally, a control (test water without test item) was tested in parallel

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
In each treatment and the control, 3 fish were tested in about 3 liters of test medium in a glass-aquarium. The mean body length of the test fish at the start of the test was 2.8 ± 0.22 cm (mean ± standard deviation). The mean body wet weight was 0.17 ± 0.03 g. The fish were acclimated to the conditions of the test according to the test guidelines.

Study design

Test type:
Water media type:
Limit test:
Total exposure duration:
96 h

Test conditions

Test temperature:
22 degrees C
Dissolved oxygen:
8.4 mg/L at start; 8.2 to 8.4 mg/L at 96 hours
Nominal and measured concentrations:
Nominal 1.0, 10 and 100 mg/L
Details on test conditions:
A 16-hour light to 8-hour dark photoperiod, light intensity between 50 and 500 Lux
Reference substance (positive control):

Results and discussion

Effect concentrations
96 h
Dose descriptor:
no effects observed
Effect conc.:
100 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: No effects on mortality or mobility
Details on results:
There were no effects on mortality or mobility observed in any of the test concentrations.

Applicant's summary and conclusion

Based on this study, chlorinated methyl esters are not acutely toxic to fish at loading level up to 100 mg/L. This concentration is well above the expected water solubility limit for this substance.
Executive summary:

A 96 hour study of chlorinated methyl esters in Zebra fish was conducted using water accommodated fractions (stirred for 96 hours) at nominal concentrations of 1, 10 and 100 mg/L. There were no effects on mobility of mortality at at concentration. The LC50 is >100 mg/L. The EC0 for this study was 100 mg/L.