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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2019
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019
Report date:
2019

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Remarks:
this test was not carried out initially to meet the REACH registration requirement, hence the fact that it is not carried out under GLP.

Test material

Constituent 1
Chemical structure
Reference substance name:
Dioctadecyl ether
Cas Number:
6297-03-6
Molecular formula:
C36H74O
IUPAC Name:
Dioctadecyl ether
Constituent 2
Chemical structure
Reference substance name:
Dieicosyl ether
Cas Number:
502696-65-3
Molecular formula:
C40H82O
IUPAC Name:
Dieicosyl ether
Constituent 3
Chemical structure
Reference substance name:
Didocosyl ether
Cas Number:
77456-42-9
Molecular formula:
C44H90O
IUPAC Name:
Didocosyl ether
Constituent 4
Chemical structure
Reference substance name:
Methanesulphonic acid
EC Number:
200-898-6
EC Name:
Methanesulphonic acid
Cas Number:
75-75-2
Molecular formula:
CH4O3S
IUPAC Name:
methanesulphonic acid
Constituent 5
Chemical structure
Reference substance name:
Octadecan-1-ol
EC Number:
204-017-6
EC Name:
Octadecan-1-ol
Cas Number:
112-92-5
Molecular formula:
C18H38O
IUPAC Name:
octadecan-1-ol
Constituent 6
Chemical structure
Reference substance name:
Icosan-1-ol
EC Number:
211-119-4
EC Name:
Icosan-1-ol
Cas Number:
629-96-9
Molecular formula:
C20H42O
IUPAC Name:
icosan-1-ol
Constituent 7
Chemical structure
Reference substance name:
Docosan-1-ol
EC Number:
211-546-6
EC Name:
Docosan-1-ol
Cas Number:
661-19-8
Molecular formula:
C22H46O
IUPAC Name:
docosan-1-ol
Constituent 8
Chemical structure
Reference substance name:
betaine glycine mesylate
Molecular formula:
C6NO5
IUPAC Name:
betaine glycine mesylate
Constituent 9
Chemical structure
Reference substance name:
Octadecyl betainate mesylate
Molecular formula:
C24NO4
IUPAC Name:
Octadecyl betainate mesylate
Constituent 10
Chemical structure
Reference substance name:
Eicosyl betainate mesylate
Molecular formula:
C26NO4
IUPAC Name:
Eicosyl betainate mesylate
Constituent 11
Chemical structure
Reference substance name:
Docosyl betainate mesylate
Cas Number:
Docosyl betainate mesylate
Molecular formula:
C28NO4
IUPAC Name:
Docosyl betainate mesylate

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
SFE: Solubilized fraction or forming a homogeneous suspension in water, obtained from a loading rate in mg / L of the product to be studied.
To prepare each ESF, a quantity of product to be studied is brought into contact with test medium at the load rate tested, in a closed container, with orbital agitation for 24 hours at approximately 110 rpm, at 20 ° +/- 2 ° C.
After shaking, the vials containing the loading levels are subjected to ultrasound for 1 minute. The aqueous fraction of each loading rate (FSE) is then recovered.
The SFE are used immediately for the performance of the test but the results are expressed as loading rate.

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
225 mg/L +/- 50 mg (CaCO3)
pH:
7.8 +/- 0.5
Dissolved oxygen:
< 7 mg/L
Salinity:
Ca/Mg = 4/1 = +/-0.5
Nominal and measured concentrations:
Nominal: 90mg/L, 80mg/L, 62mg/L, 48mg/L, 37mg/L
Reference substance (positive control):
yes

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
ca. 83.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
ca. 87 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
mobility
Results with reference substance (positive control):
Positive control: K2Cr2O7
EC50-48h = 0.92mg/L (to be valid, the value should be between 0.60 mg/L and 2.1mg/L)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

The toxicity of the test item to aquatic invertebrate was performed according to the OECD 202 guideline.


The results were the following:


EC50 48h=83.8mg/L


EC50 24h=87mg/L