3-[(phenylcarbamoyl)amino]phenyl 4-methylbenzenesulfonate

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

8 May 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Version / remarks:

22 January 2001

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Specific details on test material used for the study:

- Storage conditions: The test material was stored in a dark storage place at room temperature.

Radiolabelling:

no

Test temperature:

25 °C

Details on study design: HPLC method:

EQUIPMENT
- Apparatus: High performance liquid chromatograph (No. LC-134). LC-2010 AHT (Built in ultraviolet and visible spectrophotometer) (Shimadzu) (measurement wavelength 210 nm for reference item and 258 nm for test material).
- Type, material and dimension of analytical (guard) column: Inertsil CN-3 (150 mm x 2.1 mm I.D., particle size 5 μm, GL Sciences).

MOBILE PHASES
- Type: Methanol/purified water (60/40 v/v)
- Flow rate: 0.2 mL/min

REFERENCE SUBSTANCE
- Formamide (201.1 mg, 1 mL; 10 mL Methanol),
- N, N-Dimethylbenzamide (11.0 mg, 1 mL; 10 mL Methanol)
- Atrazine (10.1 mg, 1 mL; 10 mL Methanol)
- Linuron (2.12 mg, 1 mL; 2 mL Methanol)
- 1, 2, 3, - Trichlorobenzene (10.1 mg, 0.5 mL; 10 mL Methanol)
- Phenanthrene (10.2 mg, 2 mL; 10 mL Methanol)
- DDT (11.1 mg, 2 mL; 10 mL Methanol).

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 2 μL
- Quantity of reference substances: 2 μL
- Intervals of calibration: The reference material solution was injected before and after the injection of the test material solution to confirm that the retention times of the reference material did not change. Order was: reference material solution (first time), test material solution (first time), test material solution (second time), solvent blank, reference material solution (second time).

REPETITIONS
- Number of determinations: The test material retention time was measured twice.

EVALUATION
- Calculation of capacity factors k: Calculated from the retention time of the test material, using the following equation:

k = (tR - t0) / t0

Where:
tR: Retention time of reference material (min)
t0: Dead time (min) (average of two measured values).

- Determination of the log Koc value: The adsorption coefficient of the test material was calculated using the regression equation of regression line and was given as the average value of two calculated values.

log Koc = a x log k + b

Where:

a: Slope of regression equation
b: Intercept of regression equation

Key result

Sample No.:

#1

Type:

log Koc

Value:

4.16 dimensionless

Temp.:

25 °C

Remarks on result:

other: pH not reported

Details on results (HPLC method):

log Koc of the test material was 4.16. It was judged that the test results were valid because the difference between two measured values was less than 0.25.

Transformation products:

not measured

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this study the log Koc of the test material was determined to be 4.16.

Executive summary:

The adsorption coefficient on soil and on sewage sludge of the test material was investigated in accordance with the standardised guidelines OECD 121 under GLP conditions, following the HPLC Method.

The study was carried out using an HPLC system. The mobile phase was HPLC Methanol/purified water (60/40 v/v). The reference substances used to create the calibration curve were Formamide, N, N- Dimethylbenzamide, Atrazine, Linuron, 1,2,3- Trichlorobenzene, Phenanthrene and DDT. 

Under the conditions of this study the log Koc of the test material was determined to be 4.16.

Description of key information

The log Koc of the test material was determined to be 4.16.

Key value for chemical safety assessment

Koc at 20 °C:

14 454.4

Additional information

The adsorption coefficient on soil and on sewage sludge of the test material was investigated using the HPLC method in accordance with the standardised guidelines OECD 121 under GLP conditions. The study was awarded a reliability score of 1 in accordance with the criteria set forth by Klimisch et al. (1997).

The study was carried out using an HPLC system. The mobile phase was HPLC Methanol/purified water (60/40 v/v). The reference substances used to create the calibration curve were Formamide, N, N- Dimethylbenzamide, Atrazine, Linuron, 1,2,3- Trichlorobenzene, Phenanthrene and DDT. 

Under the conditions of this study the log Koc of the test material was determined to be 4.16.