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Physical & Chemical properties

Water solubility

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Reference
Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 2020-05-13 to 2020-06-04
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method A.6 (Water Solubility)
Version / remarks:
2008
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 105 (Water Solubility)
Version / remarks:
1995
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
column elution method
Key result
Water solubility:
0.54 mg/L
Conc. based on:
test mat.
Temp.:
20 °C
pH:
>= 6.32 - <= 7.09

The mean value and its standard deviation from five consecutive samples taken from the saturation plateau were calculated for each run. The mean concentrations calculated on two tests using different flows were compared and the repeatability was calculated, which should be better than 30 %.


Results of column 1 (flow rate 13.2 mL/h)








































Fraction



pH value



Measured concentration [mg/L]



Mean concentration


[mg/L]



Relative standard deviation (RSD) [%]



30



7.09 (24.3 °C)



0.589



0.523



10.3



32



6.39 (24.5 °C)



0.482



34



6.36 (24.5 °C)



0.513



36



6.51 (24.5 °C)



0.566



38



6.32 (24.6 °C)



0.464



The first column yielded in a mean value of the water solubility of 0.523 mg/L with a RSD of 10.3 % which meets the criterion of < 30 %.


Results of column 2 (flow rate 16.6 mL/h)








































Fraction



pH value



Measured concentration


[mg/L]



Mean concentration


[mg/L]



Relative standard deviation (RSD)


[%]



44



6.78 (21.4 °C)



0.553



0.566



3.7



46



6.52 (21.7 °C)



0.573



48



6.46 (21.6 °C)



0.557



50



6.59 (21.5 °C)



0.550



52



6.48 (21.6 °C)



0.600



The second column yielded in a mean value of the water solubility of 0.566 mg/L with a RSD of 3.7 % which meets the criterion of < 30 %.


Results of column 3 (flow rate 16.6 mL/h, blank)








































Fraction



pH value



Measured concentration


[mg/L]



Mean concentration


[mg/L]



Relative standard deviation (RSD)


[%]



44



6.41 (21.5 °C)



--



--



--



46



6.33 (21.4 °C)



--



48



6.62 (21.7 °C)



--



50



6.47 (21.6 °C)



--



52



6.74 (22.0 °C)



--



The third column showed no test item in the water or any signals in the UV/VIS-measurement.


Summarised results of column 1 and 2






















Column



Concentrations


[mg/L]



Mean measured concentration


[mg/L]



Relative standard deviation (RSD)


[%]



1



0.523



0.545



4.0



2



0.566



The two columns with different flow rates yielded in a mean value of the water solubility of 0.545 mg/L with a RSD of 4.0 % which meets the criterion of < 30 %.

Conclusions:
The water solubility of the test item was determined to be 0.54 mg/L at 20 °C and a pH around 6.7.
Executive summary:

The water solubility was determined according to Regulation (EC) No 440/2008, method A.6 and OECD Guideline No. 105, adopted on 27th July 1995 using the column elution method with external UV/Vis analysis. Two columns were loaded with 18.0 mg of the test item. For the measurements the flow through the columns was started with a flow rate of approximately 13.2 mL/h (column 1) and 16.6 mL/h (column 2). Water was recirculated until equilibration was established, as defined by five successive samples whose concentrations did not differ by more than ± 30 %. The mean water solubility of the test item was determined to be 0.54 mg/L at 20 °C and a pH around 6.7.

Description of key information

The water solubility of the test item was determined to be 0.54 mg/L at 20 °C and a pH around 6.7 (reference 4.8-1).


 

Key value for chemical safety assessment

Water solubility:
0.54 mg/L
at the temperature of:
20 °C

Additional information

The water solubility was determined according to Regulation (EC) No 440/2008, method A.6 and OECD Guideline No. 105, adopted on 27th July 1995 using the column elution method with external UV/Vis analysis. Two columns were loaded with 18.0 mg of the test item. For the measurements the flow through the columns was started with a flow rate of approximately 13.2 mL/h (column 1) and 16.6 mL/h (column 2). Water was recirculated until equilibration was established, as defined by five successive samples whose concentrations did not differ by more than ± 30 %. The mean water solubility of the test item was determined to be 0.54 mg/L at 20 °C and a pH around 6.7.