Methyl (R)-2-(4-(3-chloro-5-trifluoromethyl-2-pyridyloxy)phenoxy)propionate

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Remarks:

Intraperitoneal injection

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EPA Pesticide Assessment Guidelines; Subdivision F - Hazard Evaluation: Human and Domestic Animals, Section 81-1, 1982

Deviations:

yes

Remarks:

dosed via intraperitoneal route

GLP compliance:

yes

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl (R)-2-(4-(3-chloro-5-trifluoromethyl-2-pyridyloxy)phenoxy)propionate

EC Number:

406-250-0

EC Name:

Methyl (R)-2-(4-(3-chloro-5-trifluoromethyl-2-pyridyloxy)phenoxy)propionate

Cas Number:

72619-32-0

Molecular formula:

C16H13ClF3NO4

IUPAC Name:

methyl 2-(4-{[3-chloro-5-(trifluoromethyl)pyridin-2-yl]oxy}phenoxy)propanoate

Test material form:

solid

Specific details on test material used for the study:

R Enantiomer of XRD-453 Methyl Ester
Lot # AGR 259823
Purity: 98.6%

Test animals

Species:

rat

Strain:

Fischer 344

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

corn oil

Doses:

100, 500 or 1000 mg test substance per kg body weight

No. of animals per sex per dose:

5

Control animals:

no

Statistics:

Means and standard deviations of body weights were calculated. The data were evaluated for statistical outliers by a sequential test, however, outliers were not excluded from statistical procedures if found. An approximate LD50 was obtained by nonlinear interpolation.

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

224 mg/kg bw

Based on:

test mat.

Mortality:

All males and females given 500 or 1000 mg/kg died by test day 3.

Clinical signs:

In-life observations of some rats that died consisted of lethargy, palpebral closure, lacrimation, facial/perineal soiling and labored respiration. Rats in the 100 mg/kg dose group were normal throughout the observation period.

Body weight:

All surviving rats gained weight by study termination.

Gross pathology:

Animals that died had nonspecific terminal changes consisting of perineal soiling with urine, facial soiling, visceral congestion, erosions and/or ulcers in the stomach and hemorrhage in the thymus. The origin of the blood in the urine of one male rat given 1000 mg/kg was not apparent. All male and female rats given 100 mg/kg were within normal limits.

Applicant's summary and conclusion

Conclusions:

Rat Intraperitoneal LD50 (male/female): 224 mg/kg

Executive summary:

Five Fischer 344 rats per sex received a single intraperitoneal injection of 100, 500 or 1000 mg/kg of the test substance. Parameters examined during the two-week observation period included body weights and in-life observations.

All animals were examined for gross pathologic changes. All males and females given 500 or 1000 mg/kg died by test day three. In-life observations of some rats that died consisted of lethargy, palpebral closure, lacrimation, facial/perineal soiling and labored respiration. Rats in the 100 mg/kg dose group were normal throughout the observation period and gained weight by study termination. At necropsy, animals that died had nonspecific terminal changes. One male rat in the 1000 mg/kg dose group had blood in the urine; however, the origin was not apparent. All male and female rats given 100 mg/kg were within normal limits.

The acute intraperitoneal LD50 of the test substance was 224 mg/kg for male and female rats.