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EC number: 701-270-9
CAS number: -
Mean % tissue viablity for the test article was 11%.
The dermal irritation potential of MTDID 18990 was evaluated in the
EpiDerm reconstructed human epidermal tissue model. The study was
conducted according to OECD 439. EpiDerm tissues (n=3) were exposed to
30 uL of test article (neat), DPBS (negative control) or SDS (postive
control) for 60 minutes at 37C. Following exposure, the tissues were
washed 20 times by filling the plate wells with a gentle stream of DPBS
and emptying. The plate wells were then filled with fresh media and
returned to an incubator at 37C for 24 hours. At 24 hours, the media was
replaced with fresh media and incubated for another 18 hours. Following
incubation the tissues were patted dry with a tissue and transferred to
plates containing 300 uL of MTT solution per well. The tissues were
incuabted with MTT for 3 hours. Following incubation they were washed
and the wells were filled with MTT extraction soltuion and extracted for
2 hours at room temperature. The OD at 570 nm was measured for each
solution and normalized to the mean of the negative control group to
calculate % tissue viability. MTDID 18990 -exposed tissues had %
viability values of 8, 5, and 19% for a mean tissue viability of 11%.
Based on the results of the study (11% mean tissue viability following
60 minute exposure), MTDID 18990 is a skin irritant.
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