Tris(trimethylsilyl) phosphate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source (i.e. manufacturer or supplier) and lot/batch number of test material:
Jiu Jiang Tinci Materials Techonology Co.
N200611031
- Purity, including information on contaminants, isomers, etc.:
99.28%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
Refrigeration (+2 to +8ºC)
- Stability and homogeneity of the test material in the vehicle/solvent under test conditions (e.g. in the exposure medium) and during storag:
Stable
- Stability in the medium, i.e. sensitivity of the test material to hydrolysis and/or photolysis:
Stable
- Solubility and stability of the test material in the solvent/vehicle and the exposure medium:
Soluble in water.
- Reactivity of the test material with the incubation material used (e.g. plastic ware):
Non-reactive.

Oxygen conditions:

anaerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
Sewage Treatment Plant
Eurofins Advinus Limited
Bengaluru – 560 058
India
- Laboratory culture:
The decanted effluent was preconditioned by aerating for 5 days at 21.3 to 22.7℃.
- Method of cultivation:
- Storage conditions:
at 21.3 to 22.7℃.
- Storage length:
5 days

Duration of test (contact time):

28 d

Initial conc.:

3 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
Solution Chemical Quantity (g)
A Potassium dihydrogen orthophosphate, KH2PO4 8.50
Dipotassium hydrogen orthophosphate, K2HPO4 21.75
Disodium hydrogen orthophosphate dihydrate, Na2HPO4.2H2O 33.40
Ammonium chloride, NH4Cl 0.50
These four constituents will be dissolved in water and the volume will be made up to 1 L. The pH of the solution should be 7.4+0.2


B Calcium chloride, dihydrate, CaCl2.2H2O
This will be dissolved in water and the volume will be made up to 1 L. 36.40

C Magnesium sulphate heptahydrate, MgSO4.7H2O
22.50

This will be dissolved in water and the volume will be made up to 1 L.

D Iron (III) chloride hexahydrate, FeCl3.6H2O
0.25

The mineral medium will be prepared by mixing 1 mL of solution A, B, C and D with 800 mL of water and final volume will be made up to 1 L. The pH of the mineral medium should be 7.4 ± 0.2.

TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration:
total 16 flasks
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
sealed bottles
- Measuring equipment:



SAMPLING
- Sampling frequency:
dissolved oxygen concentration analysis will be made every three or four days during the 28-day exposure period.


CONTROL AND BLANK SYSTEM
- Inoculum blank:
For blank (BL), only mineral medium with inoculum will be used.
- Toxicity control:
for preparation of toxicity control (TC), 15 mg sodium benzoate and 15 mg test item will be used to achieve test concentration of 3 mg/L.


STATISTICAL METHODS:

Reference substance:

benzoic acid, sodium salt

Remarks:

Sodium benzoate

Key result

Parameter:

% degradation (O2 consumption)

Value:

1.42

Sampling time:

7 d

Key result

Parameter:

% degradation (O2 consumption)

Value:

1.33

Sampling time:

14 d

Key result

Parameter:

% degradation (O2 consumption)

Value:

0.08

Sampling time:

21 d

Key result

Parameter:

% degradation (O2 consumption)

Value:

2.42

Sampling time:

28 d

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

According to the results, the substance is not readily biodegradable as the biodegradation fail to meet the 60% criteria.

Description of key information

According to the results, the substance is not readily biodegradable as the biodegradation fail to meet the 60% criteria.

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater