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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2000.06.20-2012.07.2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study
Qualifier:
according to guideline
Guideline:
other: OECD Test Method No. 301F, manometric respirometry test
GLP compliance:
yes
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: Lot No. JN2TE005
- Expiration date of the lot/batch: 11-12-2004

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: -20C
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
Activated sludge from municipal waste water treatment plant was applied.
The activated sludge was stabilised under continuous aeration conditions for 1 day
Duration of test (contact time):
28 d
Initial conc.:
20 mg/L
Details on study design:
TEST CONDITIONS
- Composition of medium: deionised water, Activated sludge from municipal wastewater treatment plant, test substance/reference substance/blank
- Test temperature: 22+/-11°C
- pH: No data
- Aeration of dilution water: No data
- Continuous darkness: No data

TEST SYSTEM
- Number of culture flasks/concentration:3
- Method used to create aerobic conditions:respirometer containing pressure transducer. Oxygen is consumed and CO2 generated which is absorbed in a trap containing KOH. This creates a decrease in pressure equal to the oxygen consumed. The pressure transducer detects the change in pressure initiating an analytical process by which oxygen is supplied.
- Test performed in closed vessels.
SAMPLING
- Sampling frequency: daily
- Sampling method: daily readings/measurements

CONTROL AND BLANK SYSTEM
- Toxicity control: yes
Reference substance:
other: Sodium benzoate
Preliminary study:
no data
Parameter:
% degradation (CO2 evolution)
Value:
ca. 97
Sampling time:
28 d
Parameter:
% degradation (CO2 evolution)
Value:
ca. 85
Sampling time:
12 d
Details on results:
At the end of the 10 day window covering the period from day 2 to 12, the BOD was 85%+/-2% of the ThOD for complete oxidation of the test substance. A degradation of 97 % was observed after 28 days.
The difference between extreme replicates was 6% at the end of the test, which is in accordance with the validity criteria of a maximum deviation of 20%.
Results with reference substance:
A degrdation of 91 % was observed after 14 days (sodium benzoate), which is higher than the required 60% after 14 days.

no

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
The test substance L-TEE was ready biodegradable according to the test guideline OECD 301F. A degradation of 97%+/-3% of the ThOD was observed after 28 days.
Executive summary:

The biodegradation of L-TEE was tested according to the OECD guideline 301F. A biodegradation of 85 +/-2% was observed at the end of the 10-day window (day 2 -12) and 97%+/-3% degradation was observed after 28 days. Therefore L-TEE is considered to be readily biodegradable.

Description of key information

The biodegradation of L-TEE was tested according to the OECD guideline 301F. All criteria for readily biodegradabilty were fulfilled. Therefore L-TEE is considered to be readily biodegradable

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:
freshwater

Additional information

The biodegradation of L-TEE was tested according to the OECD guideline 301F. A biodegradation of 85 +/-2% was observed at the end of the 10-day window (day 2 -12) and 97%+/-3% degradation was observed after 28 days. Therefore L-TEE is considered to be readily biodegradable.