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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2019-0909 to 2019-09-25
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Details on test material:
Test item: (1S,4R)-1-Methyl-4-(prop-1-en-2-yl)cyclohex-2-enol
CAS number: 22972-51-6
Physical state: Liquid
Storage: Room temperature
Specific details on test material used for the study:
Test item: (1S,4R)-1-methyl-4-(prop-1-en-2-yl)cyclohex-2-en-1-ol (PMDOL)
CAS number: 22972-51-6
Physical state: Liquid
Storage: room temperature

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Preparation of test solutions
The test article stock solution (nominal concentration: 1000 mg/mL) was prepared by dissolving 50 mg of test article in dilution water to a final volume of 50 mL. The stock solution was further diluted with dilution water to prepare the test article solutions of 100.0 mg/L, 50.0 mg/L, 10.0 mg/L, 1.0 mg/L, and 0.1 mg/L.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Source: Freshwater bioresource center, National Chiayi University.
Quality criteria: less than 24 hours old.

Study design

Test type:
static
Water media type:
other: reconstituted water according to OECD 202
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
140 to 250 mg/L (CaCO3)
Test temperature:
21 ± 1°C (recorded daily)
pH:
6 to 9 : measured at the beginning and end of the test in the control solution and in the highest test article concentration solution.
Dissolved oxygen:
more than 3 mg/L in the control and in the highest test article concentration solution: measured at the beginning and end of the test in the control solution and in the highest test article concentration solution.
Nominal and measured concentrations:
The test article solutions of 100.0 mg/L, 50.0 mg/L, 10.0 mg/L, 1.0 mg/L, and 0.1 mg/L.
Details on test conditions:
The test duration is 48 hours. At the start of the study each test vessels contained 40 ml of test solutions and five young daphnids. In the test four replicates total of 20 animals at each test concentration level were investigated. Immobility of daphnia was checked at 0, 24 ± 1 and 48 ± 1 hours after the expose. Any abnormal behaviors were also recorded. The daphnia were not fed during the test.

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
71.67 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In this Daphnia Test the results indicated that the test item PMDOLis hazardous to the aquatic environment and classified into the third acute toxic hazardous category [GHS Acute aquatic hazard Category Acute III. – 48 hr EC50 (for Crustacea) > 10 mg/L but ≤ 100 mg/L].