N1,N2-dimethyl-N1-{2-[methyl(propan-2-yl)amino]ethyl}-N2-(propan-2-yl)ethane-1,2-diamine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

OECD 202 (13 April 2004)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Active substance: 100%

Analytical monitoring:

yes

Details on sampling:

Chemical analysis of the test item concentration in the test solutions was performed to determine exposure concentration and stability of the test item during exposure. Substance specific analysis was performed at an external test site.
The samples were filled into glass vials, stabilised with 50% Acetonitrile and stored in the freezer (≤ -18 °C). Sampling and documentation were conducted according to the following specification:
After 0 h, 24 h and 48 h exposure, two replicates of NC, A, B, C, D, E, F and G were sampled. At 24 h, both the 24 h old test solutions and the freshly prepared test solutions were sampled. Per replicate 1 ml of each test vessel of one treatment group was taken and pooled in one glass vial to obtain 4 ml in total. To each vial 4 ml Acetonitrile were added for stabilization and samples were stored in the freezer (≤ -18 °C).
Of each sampled treatment, one of the analytical samples from 0 h, 24 h old and new and 48 h was sent to the analytical laboratory at the external test site for chemical analysis using an insulated box with thermal packs. The remaining samples were stored as retain samples in the freezer (≤ -18 °C) until finalisation of the study.
In the control, the test item was not detected. The mean measured test item concentrations in the test item treatments were 99.1 – 110.5 % of the nominal concentrations. As the mean measured test item concentrations are within ± 20 % of the nominal concentrations, according to OECD 202 (2004) and OECD 23 (2000), all results are given in relation to the nominal test item concentrations.

Vehicle:

no

Details on test solutions:

The pH was 7.9 – 8.3 in the control (required: pH 6 – 9) and 7.9 – 9.7 in the test item treatments.
The dissolved oxygen concentration was 8.2 – 8.5 mg/L in the control and 8.1 – 8.5 mg/L in the test item treatments.
The temperature during the exposure was 19.7 – 20.4 °C.

Test organisms (species):

Daphnia magna

Details on test organisms:

The test organism Daphnia magna Straus originates from the clone breeding of the Federal Environment Agency, department IV 2.4, Berlin, and has been cultivated since October 2012. The daphnids are cultured at 20 +/- 2 °C with 16 h light : 8 h dark and placed into fresh Elendt M4 medium twice a week. They are kept at approx. 10 animals / 200 mL and fed on workdays with Desmodesmus subspicatus algae corresponding to 0.1 mg C × daphnid-1 × day-1.
The test organisms used were 1.25 - 22.75 h old at the start of the test.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

The temperature during the exposure was 19.7 – 20.4 °C.

pH:

The pH was 7.9 – 8.3 in the control (required: pH 6 – 9) and 7.9 – 9.7 in the test item treatments.

Dissolved oxygen:

The dissolved oxygen concentration was 8.2 – 8.5 mg/L in the control and 8.1 – 8.5 mg/L in the test item treatments.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

65.34 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

27.85 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC10

Effect conc.:

65.54 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

The EC 50 was determined to be at 65.34 mg/L, based on mobility (72 h)

Description of key information

The EC 50 was determined to be at 65.34 mg/L, based on mobility (48 h)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

LC50

Effect concentration:

65.34 mg/L