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EC number: 411-790-5 | CAS number: 54390-87-3 KY-MA
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 9 September 1992 - 7 October 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- The study has been performed according to OECD and/or EC guidelines and according to GLP principles. Limited information available to verify the composition of the used test substance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- (1984)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,4-bis[N'-(4-methylphenyl)ureido]toluene
- EC Number:
- 411-790-5
- EC Name:
- 2,4-bis[N'-(4-methylphenyl)ureido]toluene
- Cas Number:
- 54390-87-3
- Molecular formula:
- C23H24N4O2, C24H34N4O2
- IUPAC Name:
- 3-(2-methyl-3-{[(4-methylphenyl)carbamoyl]amino}phenyl)-1-(4-methylphenyl)urea; 3-(2-methyl-5-{[(4-methylphenyl)carbamoyl]amino}phenyl)-1-(4-methylphenyl)urea; 3-{2-methyl-3-[(octylcarbamoyl)amino]phenyl}-1-(4-methylphenyl)urea
- Details on test material:
- - Name of test material (as cited in study report): KY-MA
- Substance type: light yellow solid
- Physical state: solid
- Analytical purity: 99%
- Stability: stable at least 48 hours in water
- Storage condition of test material: RT in the dark
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municiple sewage treatment plant Waterschap de Aa, Schijndel, The Netherlands
- Preparation of inoculum for exposure: The activated sludge was aerated for 4 hours aeration. After 0.5 hours settling, the supernatant was decanted to provide a significantly large volume for a 1% inoculum for each test flask - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Test temperature: 17-21 degrees C
- pH: 4.8-6.7
- pH adjusted: No
- Continuous darkness: yes - brown vessels
TEST SYSTEM
- Number of culture flasks/concentration: 1
- Test performed in closed vessels due to significant volatility of test substance:
- Details of trap for CO2 and volatile organics if used: barium hydroxide (CO2 produced in each bottle reacted with the barium hydroxide in the gas scrubbing bottle and precipitated out as barium carbonate; amount of CO2 produced was determined by titrating the remaining Ba(OH)2 with 0.05 N standardized HCl.
SAMPLING
- Sampling frequency: d2, 5, 7, 9, 12, 16, 21 and 28
Reference substance
- Reference substance:
- other: sodium acetate
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 3.8
- Sampling time:
- 28 d
- Remarks on result:
- other: concentration 10 mg/L
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 1.7
- Sampling time:
- 28 d
- Remarks on result:
- other: concentration 20 mg/L
- Details on results:
- Based on the calculated ThCO2 for the test substance, only 3.8% and 1.7% biodegradation, respectively for the low (10mg/L) and high (20 mg/L) nominal test concentrations, was observed. Therefore KY-MA was judged not readily biodegradable.
BOD5 / COD results
- Results with reference substance:
- The reference substance was biodegraded more than 60% within 12 days (i.e. 62%) and was degraded to 97% by the end of the testing period (28d). The total CO2 release within the blank was 9.4 mg CO2.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Under the conditions of the test KY-MA was not readily biodegradable.
- Executive summary:
A Modified Sturm Test (CO2 evolution) was performed according to OECD 301B. There were no protocol or other deviations and the data was judged reliable. The nominal concentrations of 10 mg/L and 20 mg/L showed only 3.8% and 1.7% degradation, respectively, via CO2 evolution. Therefore the test substance was judged not readily biodegradable.
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