1,2,4-triazole-3-thiol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,2,4-triazole-3-thiol

EC Number:

221-656-6

EC Name:

1,2,4-triazole-3-thiol

Cas Number:

3179-31-5

Molecular formula:

C2H3N3S

IUPAC Name:

4H-1,2,4-triazole-3-thiol

Test material form:

solid: crystalline

Test animals / tissue source

Species:

rabbit

Strain:

Himalayan

Test system

Vehicle:

unchanged (no vehicle)

Controls:

yes, concurrent no treatment

Amount / concentration applied:

0,1g

Duration of treatment / exposure:

one time application

Observation period (in vivo):

13 days a.appl.

Number of animals or in vitro replicates:

1 (one male, two females)

Details on study design:

0.1 g of the test substance was placed in the conjunctival sac of the right eye.Then both lids were kept closed for a short time by light finger pressure.The left eye of each animal was left untreated and served as a control. The treated eye was not rinsed.
obersvation tome point: 1, 24, 72 hours, after that ones daily till day 13.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

iris score

Basis:

animal #1

Time point:

24 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 2d

Results 2

Irritation parameter:

iris score

Basis:

animal #2

Time point:

24/48/72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 4d

Results 3

Irritation parameter:

iris score

Basis:

animal #3

Time point:

24/48 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 3d

Results 4

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

48 h

Score:

2

Max. score:

4

Reversibility:

fully reversible within: 6d

Results 5

Irritation parameter:

cornea opacity score

Basis:

animal #2

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

fully reversible within: 6d

Results 6

Irritation parameter:

cornea opacity score

Basis:

animal #3

Time point:

24/48/72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 6 d

Results 7

Irritation parameter:

chemosis score

Basis:

animal #1

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

fully reversible within: 72h

Results 8

Irritation parameter:

chemosis score

Basis:

animal #2

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 13 d

Results 9

Irritation parameter:

chemosis score

Basis:

animal #3

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 13 d

Results 10

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 13d

Results 11

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

24/48/72 h

Score:

3

Max. score:

3

Reversibility:

not fully reversible within: 13d

Results 12

Irritation parameter:

conjunctivae score

Basis:

animal #3

Time point:

24/48/72 h

Score:

3

Max. score:

3

Reversibility:

not fully reversible within: 13d

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

After the individual introduction of 0.1 g test substance into the conjunctival sac of 3 rabbits, changes occurred in the cornea, iris and conjunctiva. After application of the test substance, the cornea showed slight diffuse turbidity (rating number 1) or details of the iris were slightly shaded due to the clouding (rating number 2). The turbidity zone covered almost the entire corneal area in all animals. On the 6th day of observation, these changes had subsided. The iris showed moderate circular corneal hyperemia during the first three days of observation (rating number 1). The conjunctiva reacted in all animals with diffuse flesh-colored redness (rating number 3). In addition, there was significant swelling, which led to the partial turning of the lids or to half-closed lids (rating number 3). Redness was longest until 13th observation day demonstrable. Hypersecretion, which moistened the eyelids and hair or considerable parts of the eye, was p. appl. to observe. The irritation index is 40. According to this, 3-mercapto-1,2,4-triazole has moderately irritating properties in the rabbit eye. Systemic-toxic effects did not occur after conjunctival application of the test product. The general condition of the experimental animals was undisturbed.

Executive summary:

The substance was tested for its eye-irritating properties on rabbits.0.1 g was placed in the conjunctival sac of 3 experimental animals. Observed changes: Cornea: Slight opacity or turbidity, in which details of the iris were slightly shadowed, affected the entire corneal area. Iris: moderate circulatory corneal hyperemia. Conjunctiva: diffuse flesh-colored redness;clear Swelling, which led to the partial turning of the lids or to the half-closed lids. Other eye changes: Hypersecretion, which moistened the surrounding eyelids and hair or considerable parts around the eye. These findings were no longer than 2 weeks p.appl.demonstrated. The determined stimulus index 40. For 3-mercapto-1, 2, 4-triazole, this results in moderately irritating properties in the rabbit eye. Systemic-toxic effects were not detectable.