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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Data waiving:
study scientifically not necessary / other information available
Justification for data waiving:
other:
Cross-reference
Reason / purpose for cross-reference:
data waiving: supporting information
Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: OECD guideline study conducted under GLP
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
GLP compliance:
yes (incl. QA statement)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (Secondary effluent of city sewage treatment plant
- Preparation of inoculum for exposure: sludge filtered through filter paper (No 2)
- Pretreatment: none
Duration of test (contact time):
28 d
Initial conc.:
7.39 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium:
- Additional substrate:
- Solubilising agent (type and concentration if used): none used
- Test temperature: 19-21 degrees C
- pH adjusted: no
- CEC (meq/100 g):
- Aeration of dilution water:
- Suspended solids concentration:
- Continuous darkness: yes
- Other:


TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration: 10 - 12
- Method used to create aerobic conditions:
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance: yes (sealed containers, test substance concentration verified analytically)
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used:
- Other:
SAMPLING
- Sampling frequency: day 0, 7, 14, 21, and 28


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: no

STATISTICAL METHODS: none used
Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
28 d
Remarks on result:
other: also verified test substance concentration
Results with reference substance:
Sodium benzoate (the reference substance) attained a maximum level of biodegradation (based on the BOD) of 75 and 89% after 14 days.
Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
HFO-1234ze is not readily biodegradable.

Data source

Materials and methods

Results and discussion

Applicant's summary and conclusion