1-Propene, 1,3,3,3-tetrafluoro-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Sampling method: 100 µL taken and added to appropriate vials to maintain conconcentation
- Sample storage conditions before analysis: stored at 2 - 10 ˚C and shipped to another facility at the end of the test (stability during storage and transport shown in another study)

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Vial filled with test solutions that had been purged for 30 or 60 minutes with clean air at a rate of approximately 100 mL/min. The test vessels were filled to the rim to minimize headspace and closed with transparent lids sealed with a silicone vacuum grease. This method was shown in another experiment to result in a saturated solution.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Source: Zodiac, LU Wageningen, the Netherlands
- Length at study initiation: 2.0 +/- 0.08 cm
- Weight at study initiation (mean and range, SD): 0.21 +/- 0.03
- Method of breeding: Fi from a single parent-pair bred in UV treated water
- Feeding during test: No

ACCLIMATION
- Acclimation period: At least 12 days
- Acclimation conditions: Same as test
- Type and amount of food: Pelleted fish food
- Feeding frequency: Daily
- Health during acclimation: Less than 5% for the 7 days prior to test initiation

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

Day 0: 20.6˚C
Day 4: 21.2 - 21.3˚C

pH:

Day 0: 8.0
Day 4: 7.4 - 7.5

Dissolved oxygen:

Day 0: 6.8 - 8.8
Day 4: 5.2 - 6.8

Nominal and measured concentrations:

Nominal: 30 minute and 60 minute purge
Measured: 87 and 117 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L
- Type: Closed (with transparent lids sealed with high vacuum grease)
- Material, size, headspace, fill volume: 9.8 liter test solution in 10 liter bottle
- Aeration: No
- No. of organisms per vessel: 7
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Biomass loading rate: 0.15 g/L (7 fish per 9.8 L)
- Water parameters: Oxygen content, pH and temp measured at time 0 and end of test

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hour photoperiod daily

EFFECT PARAMETERS MEASURED: Mortality and other effects at 2.5, 24, 48, 72, and 96 hours;

Reference substance (positive control):

yes

Remarks:

pentachlorophenol

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 117 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: None
- Other adverse effects control: No

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Mortality: 100%
- LC50: 0.15 mg/L

Validity criteria fulfilled:

yes

Conclusions:

In an acute aquatic toxicity study performed in accordance with OECD TG 203, the 96 hour LC50 for carp was determined to be > 117 mg/L based on mean measured concentrations.

Description of key information

The short-term toxicity of the substance to fish was determined in a study in accordance with OECD TG 203. In this study, the 96-h LC50 value is > 117 mg/L (mean measured concentration).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 117 mg/L