Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
Details on sampling:
- Sampling method: 100 µL taken and added to appropriate vials to maintain conconcentation
- Sample storage conditions before analysis: stored at 2 - 10 ˚C and shipped to another facility at the end of the test (stability during storage and transport shown in another study)
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Vial filled with test solutions that had been purged for 30 or 60 minutes with clean air at a rate of approximately 100 mL/min. The test vessels were filled to the rim to minimize headspace and closed with transparent lids sealed with a silicone vacuum grease. This method was shown in another experiment to result in a saturated solution.
Test organisms (species):
Cyprinus carpio
Details on test organisms:
- Common name: Carp
- Source: Zodiac, LU Wageningen, the Netherlands
- Length at study initiation: 2.0 +/- 0.08 cm
- Weight at study initiation (mean and range, SD): 0.21 +/- 0.03
- Method of breeding: Fi from a single parent-pair bred in UV treated water
- Feeding during test: No

- Acclimation period: At least 12 days
- Acclimation conditions: Same as test
- Type and amount of food: Pelleted fish food
- Feeding frequency: Daily
- Health during acclimation: Less than 5% for the 7 days prior to test initiation
Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
Test temperature:
Day 0: 20.6˚C
Day 4: 21.2 - 21.3˚C
Day 0: 8.0
Day 4: 7.4 - 7.5
Dissolved oxygen:
Day 0: 6.8 - 8.8
Day 4: 5.2 - 6.8
Nominal and measured concentrations:
Nominal: 30 minute and 60 minute purge
Measured: 87 and 117 mg/L
Details on test conditions:
- Test vessel: 10 L
- Type: Closed (with transparent lids sealed with high vacuum grease)
- Material, size, headspace, fill volume: 9.8 liter test solution in 10 liter bottle
- Aeration: No
- No. of organisms per vessel: 7
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Biomass loading rate: 0.15 g/L (7 fish per 9.8 L)
- Water parameters: Oxygen content, pH and temp measured at time 0 and end of test

- Adjustment of pH: No
- Photoperiod: 16 hour photoperiod daily

EFFECT PARAMETERS MEASURED: Mortality and other effects at 2.5, 24, 48, 72, and 96 hours;
Reference substance (positive control):
Key result
96 h
Dose descriptor:
Effect conc.:
> 117 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Mortality of control: None
- Other adverse effects control: No
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Mortality: 100%
- LC50: 0.15 mg/L
Validity criteria fulfilled:
In an acute aquatic toxicity study performed in accordance with OECD TG 203, the 96 hour LC50 for carp was determined to be > 117 mg/L based on mean measured concentrations.

Description of key information

The short-term toxicity of the substance to fish was determined in a study in accordance with OECD TG 203. In this study, the 96-h LC50 value is > 117 mg/L (mean measured concentration).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
Effect concentration:
> 117 mg/L

Additional information