Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
Reaction mass of lithium sodium hydrogen 4-amino-6-({5-[(5-chloro-2,6-difluoropyrimidin-4-yl)amino]-2-sulfonatophenyl}diazenyl)-5-hydroxy-3-[(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2,7-disulfonate and lithium sodium hydrogen 4-amino-6-({5-[(5-chloro-2,6-difluoropyrimidin-4-yl)amino]-2-sulfonatophenyl}diazenyl)-5-hydroxy-3-{[4-(vinylsulfonyl)phenyl]diazenyl}naphthalene-2,7-disulfonate
EC number: 941-533-7 | CAS number: -
Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial plants: short-term (with study design considered suitable for long-term assessment)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12-04-2001 to 16-07-2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: Level 1 of German Chemical Substances Act
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- For the exposed groups, the base substrate was admixed with the test substance and quartz sand, so that the desired test concentrations were established.
- Species:
- Triticum aestivum
- Plant group:
- Monocotyledonae (monocots)
- Species:
- Lepidum sativum
- Plant group:
- Dicotyledonae (dicots)
- Species:
- Sinapis alba
- Plant group:
- Dicotyledonae (dicots)
- Test type:
- early seedling growth toxicity test
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- Day: 22-25°C
Night: minimum 18°C - pH:
- 6.6-6.8
- Moisture:
- Day: 37-72%
Night: maximum 73% - Details on test conditions:
- TEST SYSTEM
- Test container (type, material, size): black plastic flower pot
- No. of seedlings per container: five
- No. of replicates per treatment group: five
- No. of replicates per control: five
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil type: sandy loam
- Organic carbon (%): 1.18
- Maximum water holding capacity (in % dry weigth): 39
GROWTH CONDITIONS
- Photoperiod: 16 h day: 6 h night: 1 h dawn/1 h dusk
- Light intensity and quality: 54-67 Watt/m2 (approx. 15000-17000 Lux)
- Day/night temperatures: day-22-25°C/night-minimum 18°C
- Relative humidity (%): day-37-72%/night-maximum 73%
- Air flow: 2000 L/h
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
% germination and growth in terms of weight of plant
TEST CONCENTRATIONS
- Spacing factor for test concentrations: two - Nominal and measured concentrations:
- 0, 60, 120, 250, 500 and 1000 mg/kg dw soil
- Reference substance (positive control):
- no
- Key result
- Species:
- Lepidum sativum
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Key result
- Species:
- Lepidum sativum
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Key result
- Species:
- Triticum aestivum
- Duration:
- 16 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Key result
- Species:
- Triticum aestivum
- Duration:
- 16 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Key result
- Species:
- Sinapis alba
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Key result
- Species:
- Sinapis alba
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the NOECs of the test substance for seedling emergence and plant growth inhibition were determined to be as follows:
for cress (Lepidum sativum)-500 mg/kg dw based on seedling emergence;
for wheat (Triticum aestivum)-1000 mg/kg dw based on growth inhibition; and
for mustard (Sinapis alba)-500 mg/kg dw based on growth inhibition.
In addition, the EC50 values were determined to be as following:
- for cress (Lepidum sativum) - EC50 > 1000 mg/kg dw based on seedling emergence;
- for wheat (Triticum aestivum) - EC50 > 1000 mg/kg dw based on growth inhibition; and
- for mustard (Sinapis alba) - EC50 > 1000 mg/kg dw based on growth inhibition - Executive summary:
A study was conducted to evaluate the toxicity of the test substance to terrestrial plants according to OECD Guideline 208 and German Chemical Substances Act, in compliance with GLP. The test was carried out indoors under environmentally controlled conditions. The test plants were arranged underneath a polymer hood which sits in a rimmed polymer trough. The seeds of three plant species (cress, wheat and mustard) were planted in natural sandy loam soil in black flower pots and exposed to test substance concentrations of 0, 60, 120, 250, 500 and 1000 mg/kg dw soil for approximately 14 d. Seedling emergence and inhibition of growth were observed compared to the control.
Under the test conditions, the NOECs of the test substance for seedling emergence and plant growth inhibition were determined to be as follows:
- for cress (Lepidum sativum)-500 mg/kg dw based on seedling emergence,
- for wheat (Triticum aestivum)-1000 mg/kg dw based on growth inhibition,
- for mustard (Sinapis alba)-500 mg/kg dw based on growth inhibition (Enste-Diefenbach, 2001).
In addition, the EC50 values were determined to be as follows:
- for cress (Lepidum sativum) - EC50 > 1000 mg/kg dw based on seedling emergence;
- for wheat (Triticum aestivum) - EC50 > 1000 mg/kg dw based on growth inhibition; and
- for mustard (Sinapis alba) - EC50 > 1000 mg/kg dw based on growth inhibition
Reference
Description of key information
The NOECs of the test substance for seedling emergence and plant growth inhibition were determined to be as follows:
- for cress (Lepidum sativum) - 500 mg/kg dw based on seedling emergence;
- for wheat (Triticum aestivum) - 1000 mg/kg dw based on growth inhibition; and
- for mustard (Sinapis alba) - 500 mg/kg dw based on growth inhibition
Therefore, 500 mg/kg soil dw is considered the lowest NOEC value.
In addition, the EC50 values were determined to be as following:
- for cress (Lepidum sativum) - EC50 > 1000 mg/kg dw based on seedling emergence;
- for wheat (Triticum aestivum) - EC50 > 1000 mg/kg dw based on growth inhibition; and
- for mustard (Sinapis alba) - EC50 > 1000 mg/kg dw based on growth inhibition
Key value for chemical safety assessment
- Long-term EC10, LC10 or NOEC for terrestrial plants:
- 500 mg/kg soil dw
Additional information
A study was conducted to evaluate the toxicity of the test substance to terrestrial plants according to OECD Guideline 208 and German Chemical Substances Act, in compliance with GLP. The test was carried out indoors under environmentally controlled conditions. The test plants were arranged underneath a polymer hood which sits in a rimmed polymer trough. The seeds of three plant species (cress, wheat and mustard) were planted in natural sandy loam soil in black flower pots and exposed to test substance concentrations of 0, 60, 120, 250, 500 and 1000 mg/kg dw soil for approximately 14 d. Seedling emergence and inhibition of growth were observed compared to the control. Under the test conditions, the NOECs of the test substance for seedling emergence and plant growth inhibition were determined to be as follows:
- for cress (Lepidum sativum) - 500 mg/kg dw based on seedling emergence;
- for wheat (Triticum aestivum) - 1000 mg/kg dw based on growth inhibition; and
- for mustard (Sinapis alba) - 500 mg/kg dw based on growth inhibition (Enste-Diefenbach, 2001).
Therefore, 500 mg/kg soil dw is considered the lowest NOEC value.
In addition, the EC50 values were determined to be as follows:
- for cress (Lepidum sativum) - EC50 > 1000 mg/kg dw based on seedling emergence;
- for wheat (Triticum aestivum) - EC50> 1000 mg/kg dw based on growth inhibition; and
- for mustard (Sinapis alba) - EC50 > 1000 mg/kg dw based on growth inhibition
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
