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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Link to relevant study record(s)

Description of key information

The ready biodegradability of the test item was determined with non-adapted activated sludge in the Manometric Respirometry Test for a period of 28 days according to OECD guideline 301 F. The test item concentration selected as appropriate was 34 mg/L, corresponding to a ThOD of 50.7 mg O2/L(58.5 mg O2/L with nitrification)per test vessel. The oxygen was depleted by the respiration of bacteria and the degradation was followed by measuring the oxygen concentration. The biodegradation rate is therefore expressed as the percentage BOD (biological oxygen demand) and was calculated for each study day. The mean oxygen depletion in the inoculum control was 32.8 mg O2/L on day 28.

In order to check the activity of the test system, sodium benzoate was used as functional control. The pass level for ready biodegradation (≥ 60% degradation) was reached within 3 days.

In the toxicity control containing both test item and reference item, the biodegradation achieved 48% after 14 days, with 53% after 28 days. The degradation of the reference item was not inhibited by the test item.Both test item replicates reached the10 % level (beginning of biodegradation) within 6 days. The 60% threshold was not reached after 28 days. The mean biodegradation of the test item on day 28 was 23%. The validity criteria of the guideline are fulfilled. According to the guideline the test item is classified as not readily biodegradable within the 28- day period of the study.

Key value for chemical safety assessment

Biodegradation in water:
not biodegradable
Type of water:

Additional information