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There are available acute toxicity test for all of the three trophic level (daphnia, aquatic plant (Lemna Minor) and fish).
The reuslts of acute toxicity tests on daphnia does not show any toxic effects.
The results of acute toxicity test on aquatic plants (Lemna minor) does not show any toxic effects.

The results of the tests for the fish toxicity performed on similar substance showed some adverse effects with a LD50 of 77.3 mg/l.

Based on the CLP criteria, Acid Blue 113:1 needs to beclassified as Aquatic Chrionic category 3 with H412.