1-Propene, 1-methoxy-

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: A Klimisch scoring of 3 has to be assigned to the study as it omitted one Salmonella tester strain mentioned in the OECD Guideline.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

only 4 strains used

GLP compliance:

yes (incl. QA statement)

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

(E)-1-methoxyprop-1-ene

EC Number:

615-927-1

Cas Number:

7319-16-6

Molecular formula:

C4H8O

IUPAC Name:

(E)-1-methoxyprop-1-ene

Method

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA 97, 98, 100, 102

Metabolic activation:

with and without

Metabolic activation system:

S9

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

yes

Positive controls:

yes

Positive control substance:

sodium azide

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA 97, 98, 100, 102

Metabolic activation:

with and without

Genotoxicity:

negative

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

-

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Substance is not mutagenic

Executive summary:

The test substance was tested for mutagenicity using the preincubation version of the Salmonella/mammalian microsome test. The four tester strains TA97, TA98, TA100 and TA102 were employed. The metabolic activation system was prepared from the livers of phenobarbital/p-naphthoflavone treated rats. Since the test compound was rather volatile care was taken to ensure a good exposure of the bacteria by a prolonged preincubation period in tightly closed glass tubes. The highest dose levels (3.16 and 10 mg/plate) caused toxic effects. There was no increase of the number of revertant colonies after treatment with Propenylmethylether while the positive control compounds gave the expected results, verifying the sensitivity of the strains and the activity of the metabolic system. Thus it can be concluded that Propenylmethylether is not mutagenic in the Ames test under the described test conditions.