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Endpoint summary

Description of key information

Abiotic degradation; Hydrolysis

Testing was not carried out using OECD 111 (13 April 2004) and Method C.7 Abiotic degradation, Hydrolysis as a Function of pH of Commission Regulation (EC) No 440/2008 (30 May 2008). The reasons for this decision were (i) Water solubility of the test item, in which all components were dissolved, was considered to be too low to perform the test and analytical methods would not be sensitive enough to detect the test material. Additionally, the test solution would need to be half this concentration to satisfy the test requirements (ii) The test item is a complex mixture to which the test method is not ideally suited. This is because components that are unstable could have significantly different hydrolytic rates and, if stable components were also present, this could lead to a conflicting result. From information provided by the Sponsor, the main functional groups within the components of the test item were esters, and these can hydrolyse at environmentally relevant pH and temperature, especially in basic conditions. However, limited aqueous solubility is considered to reduce the significance of ester hydrolysis and the test material as a whole is not expected to hydrolyse readily at environmentally relevant pH and temperature.

Ready biodegradation

A study was performed to assess the ready biodegradability of the test item in an aerobic aqueous medium. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No 301B "Ready Biodegradability; CO2 Evolution Test referenced as Method C4 -C of Commission Regulation (EC) No 440/2008 and US EPA Fate, Transport and Transformation Test Guidelines OCSPP 835.3110 (Paragraph (m)). The test item, at a concentration of 10 mg carbon/L, was exposed to activated sewage sludge microorganisms with mineral medium in sealed culture vessels in the dark at temperatures between 20 and 25 °C for 35 days. The study was extended from 28 days to 35 days at the request of the sponsor. Biodegradation of the test item was assessed by determination of carbon dioxide produced. Control solutions with inoculum and the reference item, sodium benzoate, were used for validation purposes together with a toxicity control. The test item attained 37 % biodegradation after 28 days and 42 % biodegradation after 36 days. The test material cannot therefore be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No 301 B.

Adsorption coefficient (Koc)

The adsorption coefficient of the test item was investigated using the HPLC screening method and was designed to be compatible with OECD 121 (22 January 2001) and EU Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 (30 May 2008). The test system utilised a High Performance Liquid Chromatograph (HPLC). A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used. The adsorption coefficient was determined to be > 4.27 x 10E05 with a Log10 Koc value of > 5.63.and was found to be > 4.27 x 10E05 with a Log10 Koc value of > 5.63 (OECD 121 and EU Method C.19).