Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report Date:
2018

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid

Test organisms

Test organisms (species):
Daphnia magna

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 2.1 mg/L
Nominal / measured:
meas. (geom. mean)
Key result
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 2.1 mg/L
Nominal / measured:
meas. (geom. mean)
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 1 g/L
Nominal / measured:
nominal
Key result
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 1 g/L
Nominal / measured:
nominal

Applicant's summary and conclusion

Conclusions:
One valid experiment was performed.
The study was performed using 1 concentration at 1 g/L. For the test concentration and
the blank control, 20 daphnia were exposed to the test item for 48 hours in a static test
system. After 24 and 48 hours, the immobilised daphnia were counted.
None of the animals was immobilised in the blank control as well as in the tested concentration.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a
current reference study to assure that the test conditions are reliable.
At the start and at the end of the test, the content of the test item in the test solutions was
determined using measurement of dissolved organic carbon (DOC). Because of the poor
solubility of the test item the correlation between nominal and measured concentration was
weak. However, the measured DOC concentration at the beginning shows that test substance
was present in the test solution. Nevertheless, a much lower concentration was
measured at the end of the test which might have been caused by the presence of the test
organism and adsorption or ingestion of test item onto the daphnia.
The measured concentration based on DOC measurement was 6.3 mg/L at the beginning
of the test and 0.7 mg/L at the end of the test. Therefore, the determination of the results
was based on the nominal concentrations since the correlation between nominal and
measured concentration was very weak and the geometric mean of the measured concentrations
was given as well.
No observations were made which might cause doubts concerning the validity of the study
outcome. All validity criteria were met.
The result of the test is considered valid.
Executive summary:

One valid experiment was performed.

The study was performed using 1 concentration at 1 g/L. For the test concentration and

the blank control, 20 daphnia were exposed to the test item for 48 hours in a static test

system. After 24 and 48 hours, the immobilised daphnia were counted.

None of the animals was immobilised in the blank control as well as in the tested concentration.

Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a

current reference study to assure that the test conditions are reliable.

At the start and at the end of the test, the content of the test item in the test solutions was

determined using measurement of dissolved organic carbon (DOC). The measured concentration

based on DOC measurement was 6.3 mg/L at the beginning of the test and 0.7

mg/L at the end of the test. Therefore, the determination of the results was based on the

nominal concentrations.

Because of the poor solubility of the test item the correlation between nominal and measured

concentration was weak. However, the measured DOC concentration at the beginning

shows that test substance was present in the test solution. Nevertheless, a much

lower concentration was measured at the end of the test which might have been caused

by the presence of the test organism and adsorption or ingestion of test item onto the

daphnia.

Therefore, the biological results were based on the geometric mean of the measured concentrations

and additionally on the nominal concentration.

The following results were determined for the test item Palmitoyl grapevine shoot extract

(species: Daphnia magna).

Geometric mean of measured concentration:

48h-NOEC ≥ 2.1 mg/L

48h-LOEC > 2.1 mg/L

Nominal concentration:

48h-NOEC ≥ 1 g/L

48h-LOEC > 1 g/L