Registration Dossier
Registration Dossier
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EC number: 444-240-8 | CAS number: -
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Translated from Japanese
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- not specified
- Remarks:
- Study translated from Japanese
Test material
- Reference substance name:
- -
- EC Number:
- 444-240-8
- EC Name:
- -
- Cas Number:
- 302933-83-1
- Molecular formula:
- C55-n H95.5-2n N7 013 Na1.5 (n-0-6) molecular weight: 1097-l4n (n=0-6)
- IUPAC Name:
- Sodium surfactin
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Lot no SF-S02
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A stock solution in water were prepared at 500 mg in 50 mL. Serial dilutions were then made to prepare test solutions ar 1, 20 and 100 mg/L.
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20±1°C
- pH:
- 7.7 to 8.1
- Dissolved oxygen:
- 8.3 to 8.5 mg/L
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 1 - <= 10 mg/L
- Details on results:
- Prepcipitation of the test substancewas observed at the 100 mg/L group after 24 hours. After 24 hours immobilization rates were 0, 20 and 30% for the 1, 10 and 100 mg/L groups.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- no
- Remarks:
- Prepcipitation of the test substancewas observed at the 100 mg/L group after 24 hours
- Conclusions:
- The immobilization rated of Daphnia magna after the 48-hour exposure with Sodium surfactin at 1, 10 and 100 mg/L groups were 0, 90 and 100% respectively. The 50%-effective concentration (EC50) of Sodium surfactin in Daphnia magna was estimated to be 1-10 mg/L after 48 -hour exposure.
- Executive summary:
The immobilization rated of Daphnia magna after the 48-hour exposure with Sodium surfactin at 1, 10 and 100 mg/L groups were 0, 90 and 100% respectively. The 50%-effective concentration (EC50) of Sodium surfactin in Daphnia magna was estimated to be 1-10 mg/L after 48 -hour exposure.
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