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EC number: 274-126-1 | CAS number: 69808-32-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 10 JULY 2019 to 22 AUGUST 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- The range of temperature was under the desired range. The stock solution vas autoclaved for 20 minutes. The preculture was prepared 5 days before the test start. All these deviations met the validation criteria and were considered non-critical deviations.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- The concentrations to be tested are based on non GLP pre-test. Replicates: 6 replicates for blank control, 6 replicates for solvent control, 3 replicates for each treatment. Duration: 72 hours, Lighting 4440-8880 lux, Temperature 20.3-23.6ºC
- Details on sampling:
- - Concentrations: 0.1 µg/L nominal concentration
- Sampling method:
- Sample storage conditions before analysis: - Vehicle:
- no
- Remarks:
- The solubility of the test item in test medium is < 0.1 µg/L (see validation report VB18062903G926). Therefore, this study was performed as a limit test at 0.1 µg/L to show that there is no toxicity at the LOQ (limit of quantification) of the analytical m
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus
- Strain: 86.81
- Source (laboratory, culture collection): MBM Sciencebridge GmbH (Intitut für Pflanzenphysiologie of Universität Göttingen)
- Age of inoculum (at test initiation): 2016
- Method of cultivation: permanetn culture on solid agar at 2-8ºC
ACCLIMATION
- Acclimation period: 96h
- Culturing media and conditions (same as test or not): nutrient medium and incubated
- Any deformed or abnormal cells observed: none - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- The test vessels were filled with 45 ± 1 mL of the respective test solution and incubated
open (covered with perforated plastic foil acting as a stopper) for 72 hours, shaken on an
orbital shaker to keep the algae in suspension. - Test temperature:
- 20.3 – 23.6 °C
- pH:
- Blank control 0h -7.1 72h-8.5
Solvent control 0h-7.1 72h-8.7
Test item 0.1 µg/L 0h-7.1 72h-8.6 - Nominal and measured concentrations:
- 0.1 µg/L nominal concentration
0.1 µg/L measured concentration - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass flasks total volume 65 mL
- Type (delete if not applicable): open (covered with perforated plastic foil acting as a stopper)
- Material, size, headspace, fill volume: 45 ± 1 mL of the respective test solution
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density: 2.4 *10^3 cells/mL
- Control end cells density:
- No. of organisms per vessel: BLANK CONTROL 2380 CN/mL (0h), 10510 CN/mL (24h), 88660 CN/mL (48h), 439893 CN/mL (72h); SOLVENT CONTROL 2380 CN/mL (0h), 10457 CN/mL (24h), 83270 CN/mL (48h), 388733 CN/mL (72h), TEST ITEM 2380 CN/mL (0h), 10753 CN/mL (24h), 82847 CN/mL (48h), 412993 CN/mL (72h)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates):6
- No. of vessels per vehicle control (replicates):6
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH:
- Photoperiod:72h
- Light intensity and quality: 4440 – 8880 lux
- Salinity (for marine algae):
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter
- Chlorophyll measurement:
- Other:
TEST CONCENTRATIONS
- Spacing factor for test concentrations: according pre-test
- Justification for using less concentrations than requested by guideline: poor solubility of the test item
- Range finding study
- Test concentrations: 0.1 µg/L measured concentration
- Results used to determine the conditions for the definitive study: 0.1 µg/L - Reference substance (positive control):
- yes
- Remarks:
- The 72h-EC50 values of potassium dichromate were determined in a separate reference test. For the estimation of the 72h-EC50 values of the positive control, the fits showed sufficient statistical correspondence of the data with the dose-response-equation.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other:
- Remarks:
- 72h EyC50
- Effect conc.:
- > 0.1 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Determinable
- Remarks:
- The concentration of test substance which results in a 50 percent reduction in growth rate (ErC50) relative to the control within 72hrs exposure. It is regarded as acute endpoint.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other:
- Remarks:
- 72h ErC50
- Effect conc.:
- > 0.1 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Determinable
- Remarks:
- Yield inhibition
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 0.1 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other:
- Remarks:
- Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 0.1 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.1 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other:
- Remarks:
- Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.1 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): none
- Unusual cell shape: none
- Colour differences: none
- Flocculation: none
- Adherence to test vessels: none
- Aggregation of algal cells: none
- Other:
- Any stimulation of growth found in any treatment: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: none - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50:
- Other:
ErC50 = 0.66 mg/L
EyC50 = 0.40 mg/L
- Reported statistics and error estimates:
- Mean and Standard Deviation of Cell Number/mL
- Validity criteria fulfilled:
- yes
- Conclusions:
- One valid experiment was performed.
The solubility of the test item in test medium is < 0.1 µg/L (see validation report
VB18062903G926). Therefore, this study was performed as a limit test at 0.1 µg/L to show
that there is no toxicity at the LOQ (limit of quantification) of the analytical method. Acetone was used to spike the test solution. A stock solution containing 1 mg/L in acetone was
prepared. For preparing the treatment 100 µL/L stock solution was used.
For the solvent control, acetone in a concentration of 100 µL/L was used.
Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours
with an electronic particle counter. Growth rate µ and the yield2 were determined from the
cell number at the respective observation times.
In comparison to the blank control, a slight effect can be seen with the solvent control.
Therefore, the results of the treatment are related to the solvent control.
Since no inhibition of algae growth has occurred, no statistical evaluation is carried out.
At the beginning and at the end of the test, the content of the test item in the test solutions
was determined using LC-MS-MS–determination. At the beginning of the test, the test item
could be detected very well. At the end of the test no test item could be detected. Therefore the results are related to the nominal values.
The 72h-EC50 values of potassium dichromate were determined in a separate reference
test. For the estimation of the 72h-EC50 values of the positive control, the fits showed sufficient statistical correspondence of the data with the dose-response-equation. The values
were within the range of the laboratory.
The pH of the blank control should not fluctuate by more than 1.5 units. The change was
1.4 units in the blank control.
All validity criteria were met.
No observations were made which might cause doubts concerning the validity of the study
outcome. The result of the test can be considered valid.
Reference
6.2 Positive Control
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a separate reference test.
6.2.1 Biological Results of the Reference Study (201901R301)
The results of the last study with the positive control K2Cr2O7 are presented in the following
table. The study was performed under GLP conditions in February 2019.
Table 6.2-a Biological Results of K2Cr2O7
Parameter | Value | 95% confidence interval |
72h ErC50 | 0.66 mg/L | 0.64 – 0.69 mg/L |
72h E yC50 | 0.40 mg/L | 0.38 – 0.41 mg/L |
Description of key information
According to OECD 201 resp. EU c.3
The solubility of the test item in test medium is < 0.1 µg/L (see validation report
VB18062903G926). Therefore, this study was performed as a limit test at 0.1 µg/L to show that there is no toxicity at the proposed LOQ (limit of quantification) of the analytical method. Acetone was used to spike the test solution. A stock solution containing 1 mg/L in acetone was prepared. For preparing the treatment 100 µL/L stock solution was used.
For the solvent control, acetone in a concentration of 100 µL/L was used.
Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate µ and the yield1 were determined from the cell number at the respective observation times.
In comparison to the blank control, a slight inhibition can be seen with the solvent control.
Therefore, the results of the treatment are related to the solvent control.
Slight stimulation of algal growth could be observed in the treatment compared to the solvent control. The test item showed no toxicity. Since no inhibition of algae growth has occurred, no statistical evaluation was carried out.
At the beginning and at the end of the test, the content of the test item in the test solutions was determined using LC-MS-MS–determination. At the beginning of the test, the test item could be detected very well. At the end of the test no test item could be detected. Therefore the results are related to the nominal values
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.1 µg/L
- EC10 or NOEC for freshwater algae:
- 0.1 µg/L
Additional information
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