[No public or meaningful name is available]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 March 2018 to 09 April 2018

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

2008

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

The test item is listed as ATHC (CAS # 24817-92-3) and is pale yellow slightly viscous liquid obtained from the Sponsor, Vertellus, with 99.4% purity and stored at room temperature in the dark.

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

The test was carried out using juvenile rainbow trout (Oncorhynchus mykiss). Fish were obtained from Brow Well Fisheries Limited, Hebden, near Skipton, Yorkshire, UK and maintained in-house since 09 March 2018. Fish were maintained in a glass fiber tank with a "single pass" water renewal system. Fish were acclimatized to test conditions from 19 March 2018 to 26 March 2018. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

test water was partly softened giving water a total hardness of 140 mg CaCO3/L

Test temperature:

water temperature of 14 ˚C to 15 ˚C

pH:

7.1 to 8.2

Dissolved oxygen:

9.3 mg O2/L to 11.6 O2/L

Nominal and measured concentrations:

A preliminary media preparation trial indicated that a saturated solution method of preparation was appropriate for this test item. Based on the results of the range-finding test a limit test was conducted at a concentration of 100% v/v saturated solution which corresponds to an initial loading rate 50 mg/L.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

EL50

Effect conc.:

> 50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other:

Remarks:

Test solution prepared with a loading rate of 50 mg/L resulting in a 100% v/v saturation solution.

Key result

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 100 other: % v/v saturated solution

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other:

Remarks:

100% v/v saturated solution prepared from a test solution with a loading rate of 50 mg/L.

Duration:

96 h

Dose descriptor:

NOELR

Effect conc.:

50 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other:

Remarks:

Test solution prepared with a loading rate of 50 mg/L resulting in a 100% v/v saturation solution.

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 100 other: % v/v saturated solution.

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other:

Remarks:

100% v/v saturated solution prepared from a test solution with a loading rate of 50 mg/L.

Validity criteria fulfilled:

yes

Remarks:

Control mortality 0 fish (protocol ≤ 10 %); Dissolved O2 above 60% (protocol >60%); pH 7.1 to 8.2 (protocol not vary > 1 unit).

Conclusions:

The test substance did not cause toxic effects to the freshwater fish rainbow trout (Oncorhynchus mykiss) in a limit test with a 100% v/v saturated solution after 96 h of exposure; therefore, there were no toxic effects at saturation. The effects concentrations were based on the nominal test concentration of 100% v/v saturated solution which was equivalent to an initial nominal loading rate of 50 mg/L. The effects concentrations were reported both as 48-h LC50 > 100% v/v saturated solution and 48-h EL50 >50 mg/L. The no effects concentrations were reported both as 48-h NOEC of 100% v/v saturated solution and 48-h NOELR of 50 mg/L.

Description of key information

The key study was a semi-static acute fish toxicity test on the effects of the test substance on the freshwater rainbow trout (Oncorhynchus mykiss) following OECD Guideline 203. The test substance did not cause toxic effects to the test organism in a limit test with a 100% v/v saturated solution after 96 h of exposure; therefore, there were no toxic effects at saturation. The effects concentrations were based on the nominal test concentration of 100% v/v saturated solution which was equivalent to an initial nominal loading rate of 50 mg/L. The effects concentrations were reported both as 48-h LC50 > 100% v/v saturated solution and 48-h EL50 >50 mg/L. The no effects concentrations were reported both as 48-h NOEC of 100% v/v saturated solution and 48-h NOELR of 50 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

50 mg/L