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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. certificate)
Details on sampling:
Samples were collected at the beginning and at the end of exposure period.
Vehicle:
no
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Rainbow Trout (Oncorhynchus mykiss) were used in this study. These organisms were obtained from Aquatic Research Organisms and held at BMI for 12 days before they were used in the tests. All test organisms were between 4.5 and 5.5 cm at the start of the test.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
Blacksburg, Virginia municipal tapwater was dechlorinated using activated carbon, deionized using mixed bed deionization tanks to a resistivity of > 1 megohm/cm, and polished with a Millipore, Milli Q UV Plus and 0.2 micron filter to a final resistivity of > 18 megohm/cm. This is referred to as BMI DI water and was used to prepare MHRW using EPA guidelines (e.g., EPA/600/4-90/027F).
Test temperature:
3-17 + 1°C
Dissolved oxygen:
The dissolved oxygen concentration was at least 60 percent of the air saturation value throughout the test
Nominal and measured concentrations:
Sample recovery ranged from 90.6% (1000 mg/L) of the nominal concentration to 110.6% (62.5 mg/L) of the nominal concentration.
Details on test conditions:
1. Test type: Static
2. Renewal of test solutions: Not Renewed
3. Temperature (°C): 13-17 + 1°C
4. Light quality: Ambient laboratory illumination
5. Light intensity: 50-100 foot candles (ambient laboratory levels)
6. Photoperiod: 16 hr light/ 8 hr dark
7. Size of test vessel: 4000 ml
8. Volume of test solution: >/= 4000 ml
9. Length of test organism: 5.0 + 1.0 cm
10. Number of organisms per test vessel: 10
11. Number of replicates per concentration: 2
12. Total number of organisms per conc.: 20
13. Feeding regime: Not fed
14. Aeration: None
15. Dilution Water: MHRW
16. Test duration: 96 hours
17. Test concentrations: Five product concentrations and a control
18. Dilution series: 0.1 dilution series for range-finding test 0.5 dilution series for definitive test
19. Endpoint: Mortality (LC50)
20. Sampling and sample: Fresh solutions prepared for range finding and definitive testing
21. Test acceptability criterion: < 10% organism mortality in controls
Reference substance (positive control):
yes
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
282.69 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The 96 hour survival ranged from 0% (500 and 1000 mg/L) to 95% (0 and 125 mg/L). Table 3 presents a summary of the survival data obtained.

Table3: Percent Survivability of the Rainbow Trout

Treatment

mg/L

0 H

24 H

48 H

72 H

96 H

0

100

100

100

100

95

62.5

100

100

100

100

80

125

100

100

100

100

95

250

100

100

100

85

65

500

100

100

30

0

0

1000

100

0

0

0

0

Validity criteria fulfilled:
yes
Conclusions:
Acute fish toxicity of benzylated polyamine was determined using OECD 203 Method. The LC50 value calculated was 282.69 mg/L, the 95% confidence limits were 236.81 and 337.45 mg/L.
Executive summary:

Acute fish toxicity of benzylated polyamine was determined using OECD 203 Method. The LC50 value calculated was 282.69 mg/L, the 95% confidence limits were 236.81 and 337.45 mg/L.

Description of key information

Acute fish toxicity of benzylated polyamine was determined using OECD 203 Method. The LC50 value calculated was 282.69 mg/L, the 95% confidence limits were 236.81 and 337.45 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
282.69 mg/L

Additional information