Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 279-075-9 | CAS number: 79135-28-7
Endpoint summary
Administrative data
Description of key information
Aquatic invertebrates: EC50 (48h) = 63.7 mg/l
Aquatic plants: ErC50 (7d) = 119.829 mg/l based on frond number
Additional information
The assessment on aquatic toxicity was based on data on Acid Brown 303 and on a structural analogue, i.e. Similar Substance 01.
Details on the read-across are available in section 13.
Short-term toxicity to fish
An
old study on Acid Brown 303 reported an LC50 (48h) above 1400 ppm
wt/vol. Due to the lack of details, the reliability of such study could
not be ascertained, thus it was not used for classification purposes.
Short-term toxicity to aquatic invertebrates
Similar Substance 01 was tested for acute toxicity to Daphnia magna, according to OECD guideline 202.
The EC50 value at 48 hours was 63.7 mg/l.
Toxicity to aquatic plants
Acid Brown 303 was tested for Lemna sp. according to OECD guideline 221 over a period of 7 days (168 hours) under static conditions.
The ErC50 value for growth rate based on frond number was found to be 119.829 mg/l.
Toxicity to microorganisms
Acid Brown 303 was tested according to OECD guideline 303 and caused no inhibition of the respiration rate at the highest tested concentration. Accordingly, IC50 (3h) was greater than 100 mg/l.
Justification for classification
Within the CLP Regulation (EC 1272/2008), classification for acute and chronic aquatic toxicity is based on data on fish, crustacea and algae or other aquatic plants. In particular, classification for acute toxicity applies when LC50 (96h) on fish, and/or, EC50 (48h) on crustacea and/or ErC50 (72 or 96h) on algae is ≤ 1 mg/l.
In case of lack of adequate chronic toxicity data, classification for chronic toxicity is based on acute toxicity data. In particular, classification in:
- category chronic 1 applies for acute toxicity data ≤ 1 mg/l;
- category chronic 2 applies for acute toxicity data between 1 and 10 mg/l;
- category chronic 3 applies for acute toxicity data between 10 and 100 mg/l.
Based on the available data on crustacea and aquatic plants, the substance is classified as category chronic 3 (H412), according to the CLP Regulation n. 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
