Sulphoethyl methacrylate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jun 2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Batch No. 0066758

Analytical monitoring:

yes

Details on sampling:

At least duplicate samples from the freshly prepared test media of the only test concentration and the control will be taken.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentration during the test period, the concentration in the test vessels will be sampled at least in duplicate at the end of the test (after the 48 hours test period).
All samples will be diluted by a factor of 2 with acetonitrile.
Additional samples of the control and the dilution solvent will be taken at each sampling without any sample treatment.

Vehicle:

no

Details on test solutions:

The only test item concentration will be nominal 100 mg test item/L. Additionally; a control will be tested in parallel (test water without addition of the test item). Thus a limit test will be performed in accordance with OECD Guideline 202 to demonstrate that the test item has no toxic effect on the test animals up to this concentration.
In the control, test water will be used without addition of the test item.
Before test initiation, the test medium of the only test concentration of nominal 100 mg test item/L will be prepared. The test item will be dissolved or dispersed into the test water as homogeneously as possible.
The test medium will be prepared just before introduction of the daphnids (= start of the test).

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magna (Straus), clone 5
Age at Test Start: Less than 24 hours old
Origin: The daphnids introduced in the test will be taken from ibacon's in-house laboratory culture.
Breeding Conditions: The daphnids are bred in the laboratories of ibacon under similar temperature and light conditions as used in the test. The cultivation of the parental daphnids is performed in Elendt M4 medium. The test organisms will not be first brood progeny. The daphnids in the stock culture are fed at least on all working days with green algae (Desmodesmus subspicatus) freshly grown in the laboratories of ibacon.
Reference Item: For the evaluation of the quality of the Daphnia clone and the consistency of the experimental conditions, the reference item potassium dichromate is tested at least twice a year to demonstrate satisfactory test conditions.
Acclimatisation: Is not necessary since the breeding is performed in the same water.

Test type:

static

Water media type:

other: Reconstitued Water (Elendt "M4")

Limit test:

yes

Total exposure duration:

48 h

Hardness:

Calculated Water hardness: 2.5 mmol/L (= 250 mg/L) as CaCO3

Test temperature:

18 °C - 22 °C with a maximum deviation of ± 1 °C within this range.

pH:

pH 6 – 9

Dissolved oxygen:

>3 mg/L

Salinity:

Calculated Alkalinity: 0.9 mmol/L

Details on test conditions:

1.1 Test Conditions
Test Environment: Controlled environment room
Measurement of pH, Dissolved Oxygen and Water Temperature:
The water temperature, pH-values and dissolved oxygen concentrations will be determined at test start and test end in each treatment group.
pH-Value of Test Medium: pH 6 – 9
Dissolved Oxygen Concentration:  3 mg/L
Water Temperature: 18 °C - 22 °C with a maximum deviation of ± 1 °C within this range.
Measurement of Light Intensity: The light intensity will be measured at least once during the test.
Light Regime: 16 h light : 8 h dark
Light Intensity: Not exceeding 1500 lux
Recording: Test conditions will be recorded with suitable instruments and documented in the raw data.

Reference substance (positive control):

yes

Remarks:

Control: Reconstituted water

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

The toxic effect of the test item 2-SEM to Daphnia magna was assessed in a static limit test. The 48-hour EC50 value was calculated to be >100 mg test item/L.
The initial concentrations and the maintenance of the exposure concentrations during the test were verified in the analytical part.

Description of key information

The toxic effect of the test item 2-SEM to Daphnia magna was assessed in a static limit test. The 48-hour  EC50 value was calculated to be >100 mg test item/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L