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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
21/02/2001 - 22/03/2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to OECD-guideline OECD 301E under GLP
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report date:
2001

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
Deviations:
no
Principles of method if other than guideline:
Not relevant
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
4-amino-2-methylpyrimidine-5-methylamine
EC Number:
202-384-7
EC Name:
4-amino-2-methylpyrimidine-5-methylamine
Cas Number:
95-02-3
Molecular formula:
C6H10N4
IUPAC Name:
5-(aminomethyl)-2-methylpyrimidin-4-amine
Details on test material:
- Name of test material (as cited in study report): Grewe-diamine
- Stability under test conditions: stable
- Storage condition of test material: room temperature
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 128 - 132°C
- Water solubility (under test conditions): 430 mg/L (20°C)
- Stability of test material at room temperature: stable

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, predominantly domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): secondary effluent of treatment plant
Duration of test (contact time):
> 28 d
Initial test substance concentration
Initial conc.:
40 other: ppm
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium: solution a: Kaliumdihydrogenphosphate (KH2P04) 8.5 g, di-Kaliumhydrogenphosphate (K2HPOS 21,75 g
di-Natriumhydrogenphosphate-Dihydrat (Na2HP04*2H20) 33.4 g, Ammoniumchlorid (NH4CI) 0.5 g, Aqua demin. ad 1000 ml
solution b: Calciumchloriddihydrate (CaCI2*2H20) 36.4 g, Aqua demin. ad 1000 ml
solution c: Magnesiumsulfatheptahydrate (MgS04*7H20) 22.5 g, Aqua demin. ad 1000 ml
solution d: Irion(lll)chloridehexahydrate (FeCI3*6H20) 0.25 g, Dinatriumethylendiamintetraacetate (Na2EDTA) 0.4 g, Aaua demin. ad 1000 ml
solution e: Mangansulfattetrahydrate (MnS04*4H20) 39.9 mg/l, boric acid (H3BO3) 57.2 mg/l, Zinksulfatheptahydrate (ZnS04*7H20) 42.8 mg/l, Ammoniumheptamolybdate ((NH4)6(Mo7024)) 34.7 mg/l, Iron-chelate (FeCI3-EDTA) 100 mg/l, Aqua demin. ad 1000 mg/l
solution f: yeast extract 15 mg, add water up to 100 ml
test solution: solution a 10 ml, solution b 1 ml, solution c 1 ml, solution d 1 ml, solution e 1 ml, solution f 1ml, Aqua demin. ad 1000 ml
- Test temperature: 21 + 2 °C
- Continuous darkness: no


TEST SYSTEM
- Measuring equipment: Shimadzu TOC-5050A


SAMPLING
- Sampling frequency: after 2, 5, 9, 14, 20 und 28 days

CONTROL AND BLANK SYSTEM
- Abiotic sterile control: 1% Hg2Cl2-solution
- Toxicity control: Aniline - solution

Reference substance
Reference substance:
aniline

Results and discussion

Preliminary study:
Stock solution 1g/l, dilution 1:10, carbon content determined (DOC measure)
Test performance:
See results
% Degradation
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
28 d
Details on results:
Grewe-diamine is not readily biodegradable

BOD5 / COD results

Results with reference substance:
Reference substance aniline: degradation 95% after 5 days

Any other information on results incl. tables

No additional remarks

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The substance was found to be not readily biodegradable.
Executive summary:

Grewe-diamine was tested for its ready biodegradability in the modified OECD screening test.

The study procedure was based on OECD guideline No. 301 E, July 17, 1992.

The test substance was added in a quantity of 40 ppm DOC to the test medium.

The inoculum was derived from the secondary effluent of a treatment plant. Anillin was used as reference substance. The observed biodegradability was 0% after 28 days.

The criterion for ready biodegradability (at least 70% removal of DOC in a 10 -day window) was therefore not met in these experiment. The substance was found to be not readily biodegradable.