2-(2-(4-methyl-3-cyclohexen-1-yl)propyl)cyclopentanone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20 March 1989 through 21 December, 1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Draft 1988

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1984

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Nectaryl :
- Identification - Nectaryl - LRG 1371
- Batch No. - AS 1241001

Analytical monitoring:

no

Vehicle:

yes

Remarks:

Ethanol (at a concentration of 10 mg/L in preliminary range-finder study and 11.48 mg/L in definitive study)

Details on test solutions:

A total of 8 test solutions were prepared, a Solvent Control and a Blank Control system.

The test solutions and Solvent Control contained a total of 11.84 mg of Ethanol per litre as co-solvent.

Test solution concentrations (in mg of Nectaryl per litre) : 1.50, 2.25, 3.38, 5.10, 6.00, 6.80, 7.65, 11.48 mg Nectaryl/L.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Brachydanio rerio (Hamilton-Buchanan) - all fish obtained from a single batch.

- Supplier : Société d'Elevage Piscicole Contrôlé, La Grande Rivière, 69490 Saint-Forgeux, France;
- Size : 3.4 to 4.0 cm
- Acclimitasition : Tank of approximately 200 L in reverse osmosed water with further remineralisation. Diet - Tetra-Min, ad libitum on a daily basis. Feeding stopped 24 hours before start of the study. Period of acclimatisation of 12 days before ths tart of the study, with a mortality less than 5% observed during a 7 day period preceding the study.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

125 mg CaCO3/L

Test temperature:

20.0 to 22.2 °C

pH:

pH range 6.27 to 7.56

Dissolved oxygen:

Dissolved oxygen was maintained at > 72 % of the air saturation. The test tanks were aerated.

Nominal and measured concentrations:

The Nominal Test concentrations tested were : 1.50, 2.25, 3.38, 5.10, 6.00, 6.80, 7.65, 11.48 mg Nectaryl/L

A Solvent Control (containing 11.48 mg Ethanol/L) and a Blank Control (no Ethanol) were run in parallel.

Details on test conditions:

- 10 fish per treatment group were exposed.
- No feeding performed during 96 hour exposure period.
- Tanks were cleaned daily.
- Semi-static renewal on a 24-hour basis.
- Light/Dark period : 12 hours/12 hours.
- Loading Rate - max. 1g/L
- Observation for Effects : Mortality - recorded at 24, 48, 72 and 96 hours; Sub-Lethal Effects Observations - Any abnormal behaviour including swimming, respiration, pigmentation, loss of equilibrium was recorded 2.5 hours after the start of the exposure and at every 24 hours, thereafter.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

5.47 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% Confidence Limits : 4.48 - 6.69 mg/L

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

2.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

7.65 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

6.53 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% Confidence Limits : 6.01 - 7.09 mg/L

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

6.11 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% Confidence Limits : 5.70 - 6.56 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The 96-hour LC50, LC0 and LC100 for Brachydanio rerio exposed to Nectaryl was determined to be, respectively :
5.47 mg/L, 2.25 mg/L and 7.65 mg/L.

Executive summary:

The 96-hour LC50, LC0 and LC100 for Brachydanio rerio exposed to Nectaryl was determined to be, respectively :

5.47 mg/L, 2.25 mg/L and 7.65 mg/L.

Description of key information

The 96-hour LC50, LC0 and LC100 for Brachydanio rerio exposed to Nectaryl was determined to be, respectively :

5.47 mg/L, 2.25 mg/L and 7.65 mg/L.

A further acute fish toxicity study, which was performed on O. latipes as a pre-study for an aqueous exposure Fish-BCF study, has been reported as a supporting study : 96 hour LC50 = 3.21 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

5.47 mg/L