5-(3-Phenylpropioloyl)-1,3-isobenzofurandione

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23/04/2014 to 26/05/2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

not specified

GLP compliance:

yes

Type of method:

flask method

Specific details on test material used for the study:

No further details specified in the study report.

Key result

Water solubility:

< 0.01 mg/L

Conc. based on:

test mat.

Incubation duration:

72 h

Temp.:

20 °C

pH:

3.64

Details on results:

The results of solubility determinations made after storing samples for 24, 48 and 72 hours at 30 ° C, and subsequently maintained for 24 hours at 20 ° C, are kept under the limit of quantification, so the overall value of solubility at 20 C is < 0.01 mg / l. After the test, the pHfinal of the samples has reached 3.6-3.9.

Conclusions:

The overall value of solubility at 20 C is < 0.01 mg / l.

Executive summary:

In this method, the substance is dissolved in water at 30 C. The initial pH of the water was 7. When saturation is achieved the mixture is cooled and kept at the test temperature, 20 C, stirring as long as necessary to reach equilibrium. Subsequently, the mass concentration of the substance in the aqueous solution, which must not contain any undissolved particles, is determined by a suitable analytical method.

Water solubility at 20 ± 0.5 °C was determined according to procedure PEBPL/MUNLAB/26 (guideline EEC A6 water solubility) and employing the flask method. Quantitative determination was DAD-HPLC at 254 nm with the following results.

 

Water solubility at 20 °C

Sample number	pH final / Sample Temperature	Water solubility at 20 °C (mg/l)
Solubility 24h	3.78 / 20.1 °C	< 0.01
Solubility 48h	3.87 / 20.5 °C	< 0.01
Solubility 72h	3.64 / 23.0 °C	< 0.01

 

The results of solubility determinations made after storing samples for 24, 48 and 72 hours at 30 °C, and subsequently maintained for 24 hours at 20 °C, are kept under the limit of quantification, so the overall value of solubility at 20 °C is < 0.01 mg / l. After the test, the pH final of the samples has reached 3.6-3.9.

Key value for chemical safety assessment

Water solubility:

0.01 mg/L

at the temperature of:

20 °C

Additional information

In this method, the substance is dissolved in water at 30 C. The initial pH of the water was 7. When saturation is achieved the mixture is cooled and kept at the test temperature, 20 C, stirring as long as necessary to reach equilibrium. Subsequently, the mass concentration of the substance in the aqueous solution, which must not contain any undissolved particles, is determined by a suitable analytical method.

 

Water solubility at 20 °C

Sample number	pH final / Sample Temperature	Water solubility at 20 °C (mg/l)
Solubility 24h	3.78 / 20.1 °C	< 0.01
Solubility 48h	3.87 / 20.5 °C	< 0.01
Solubility 72h	3.64 / 23.0 °C	< 0.01

 

The results of solubility determinations made after storing samples for 24, 48 and 72 hours at 30 °C, and subsequently maintained for 24 hours at 20 °C, are kept under the limit of quantification, so the overall value of solubility at 20 °C is < 0.01 mg / l. After the test, the pH final of the samples has reached 3.6-3.9.