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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 05 Jun 2003 to 04 Jul 2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
GLP study performed according to OECD 305 and METI Guideline: Concentration Test on Chemical Substances in Fish
Qualifier:
according to guideline
Guideline:
other: METI Guideline: Concentration Test on Chemical Substances in Fish
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Radiolabelling:
no
Details on sampling:
- Sampling intervals/frequency for test organisms: days 0, 7, 14, 21, 26, 28
- Sampling intervals/frequency for test medium samples: days 0, 7, 14, 21, 26, 28
- Details on sampling and analysis of test organisms and test media samples:
Water: Extraction with CH2Cl2 with further HPLC analysis.
Fish: two fish per sampling homogenised, and extracted with acetonitril/n-hexane. The analysis was performed using HPLC.
Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
In order to maintain the test material concentrations of 0.05 and 0.005 mg/L stable, Tween 80 was added as solubiliser at concentrations of 0.20 and 0.02 mg/L, respectively. The control contained Tween 80 at a concentration of 0.2 mg/L.
Test organisms (species):
Cyprinus carpio
Details on test organisms:
- Weight: 2.82 g
- Length: 6.4 cm
- Lot: 30407
- Acclimatisation: 07.04.2003 - 29.05.2003
Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
28 d
Total depuration duration:
0 d
Hardness:
250 mg/L
Test temperature:
24.6 ± 0.3 °C
pH:
7.1
Dissolved oxygen:
7.3 mg/L
Salinity:
According to guideline
Details on test conditions:
After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 0, 7, 14, 21, 26 and 28. The analytical values show that the nominal concentrations of 0.05 and 0.005 mg/L were maintained at 98 and 100%, respectively. At days 0, 7, 14, 21, 26 and 28 two fish each were removed from each test group. Body weight was determined. The fish were homogenised and extracted with acetonitrile and n-hexane. The extract was analysed with HPLC. Due to the low bioaccumulation potential, no depuration phase was required.
Nominal and measured concentrations:
Nominal concentration 0.05 mg/L: analytically measured concentrations: day 0: 0.0480 mg/L; day 7: 0.0494 mg/L; day 14: 0.0496 mg/Lday 21: 0.0492 mg/Lday 26: 0.0499 mg/Lday 28: 0.0492 mg/L
nominal concentration 0.005 mg/L: analytically measured concentrations: day 0: 0.00509 mg/L; day 7: 0.00490 mg/L; day 14: 0.00492 mg/L; day 21: 0.00509 mg/L; day 26: 0.00494 mg/L; day 28: 0.00507 mg/L
Details on estimation of bioconcentration:
according to guideline
Key result
Conc. / dose:
0.005 mg/L
Temp.:
24.6 °C
pH:
7.1
Type:
BCF
Value:
180 dimensionless
Basis:
normalised lipid fraction
Time of plateau:
21 d
Calculation basis:
steady state
Key result
Conc. / dose:
0.05 mg/L
Temp.:
24.6 °C
pH:
7.1
Type:
BCF
Value:
145 dimensionless
Basis:
normalised lipid fraction
Time of plateau:
21 d
Calculation basis:
steady state
Details on kinetic parameters:
Steady state was reached within 28 days. Due to the low uptake, no depuration phase was necessary.
Metabolites:
None.
Details on results:
- Mortality of test organisms: no effects
- Behavioural abnormalities: no effects
- Observations on body length and weight: no effects
- Other biological observations: no effects
- Bound residues forming a plateau: none
- Mortality and/or behavioural abnormalities of control: none
- Loss of test substance during test period: none
- Results with vehicle control: no effects
Validity criteria fulfilled:
yes
Conclusions:
The test material showed steady state BCF values of 145 and 180 (mean 163) at test concentrations of 0.05 and 0.005 mg/L.
Executive summary:

The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio. The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions. Two environmental concentrations of the test material of 0.05 and 0.005 mg/L were used in this study. The exposure concentration was maintained using Tween 80 as solubiliser at 98 and 100 % of the nominal concentrations. The concentrations have been verified analytically using a HPLC method with UV detection. No signs of toxicity were observed throughout the study. The analysis revealed steady state BCFs of 145 and 180. Due to the low bioaccumulation potential, no depuration phase was required. The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 145 and 180 (mean 163).

Description of key information

The test item showed no relevant bioaccumulation. The steady state BCF values were at 145 and 180 (mean 163).

Key value for chemical safety assessment

BCF (aquatic species):
163 dimensionless

Additional information

The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio. The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions. Two environmental concentrations of the test material of 0.05 and 0.005 mg/L were used in this study. The exposure concentration was maintained using Tween 80 as solubiliser at 98 and 100 % of the nominal concentrations. The concentrations have been verified analytically using a HPLC method with UV detection. No signs of toxicity were observed throughout the study. The analysis revealed steady state BCFs of 145 and 180. Due to the low bioaccumulation potential, no depuration phase was required. The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 145 and 180 (mean 163) (reference 5.3.1-1).