2H-Pyran, 2-[4'-[difluoro(3,4,5-trifluorophenoxy)methyl]-3',5'-difluoro[1,1'-biphenyl]-4-yl]-5-ethyltetrahydro-

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2008-07-24 till 2008-07-25

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Version / remarks:

2001

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

not specified

Type of method:

HPLC estimation method

Radiolabelling:

no

Test temperature:

column temperature: 25 °C

Details on study design: HPLC method:

EQUIPMENT
HPLC equipment: HPLC System Agilent 1100 series: HPLC pump G1311A, autosampler G1313A, column oven G1316A, degaser G1322A and UVA/is detector G1314A by Agilent
Column: Stationary phase: LiChrospher 100 CN 5pm by Duratec (15408-07) material: stainless steel, dimensions: 250 mm x 0 4.6 mm column temperature: 25 °C

MOBILE PHASE
Methanol, distilled water 70:30 % (v/v), isocratic elution

REFERENCE SUBSTANCES
- Identity: Acetanilide, Atrazin, Monuron, Triadimenol, Linuron, Pyrazophos, Diclofop-methyl

DETERMINATION OF DEAD TIME
- Method: by inert substance (Formamide) which is not retained by the column

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 10 µL

REPETITIONS
- Number of determinations: 2

EVALUATION
- Calculation of capacity factors k: k=0.73
- Determination of the log Koc value: logKoc = a + b * logk

Key result

Type:

log Koc

Value:

> 4.2 dimensionless

Temp.:

25 °C

Remarks on result:

other: mean value

Details on results (HPLC method):

- Retention times of reference substances used for calibration: Acetanilide 3.64 min, Atrazin 3.66 min, Monuron 3.80 min, Triadimenol 3.85 min, Linuron 4.07 min, Pyrazophos 4.38 min, Diclofop-methyl 4.47 min
- Details of fitted regression line (log k' vs. log Koc): y = 4.4x + 5.9
- Average retention data for test substance: 5.75 min

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient of the test item was determined according to the HPLC- method to be: log Koc >4.20.

Executive summary:

The objective of this study was the determination of the adsorption coefficient Koc of the test item according to the consolidated version of Guideline 67/548/EEC Annex V (Council Directive 2001/59/EC), Part C: Methods for determination of ecotoxicity.C.19.: estimation of the adsorption coefficient on soil and on sewage sludge using HPLC and OECD test guidelines OECD 121 (2001): estimation of the adsorption coefficient on soil and on sewage sludge using HPLC. The HPLC method is based on the correlation between the retention of a substance on a reversed phase column packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties and the adsorption coefficient Koc. The adsorption coefficient of the test item was derived from its retention time and the calibration graph. Two series were injected. Each series included the calibration substances and the test item. The test item was injected in-between the series of the calibration substances. The adsorption coefficient of the test item was found to be: log Koc >4.20.

Description of key information

The adsorption coefficient of the test item was determined according to the HPLC- method to be: log Koc >4.20 (reference 5.4.1 -1).

Additional information

The objective of the study was the determination of the adsorption coefficient Koc of the test item according to the consolidated version of Guideline 67/548/EEC Annex V (Council Directive 2001/59/EC), Part C: Methods for determination of ecotoxicity.C.19.: estimation of the adsorption coefficient on soil and on sewage sludge using HPLC and OECD test guidelines OECD 121 (2001): estimation of the adsorption coefficient on soil and on sewage sludge using HPLC. The HPLC method is based on the correlation between the retention of a substance on a reversed phase column packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties and the adsorption coefficient Koc. The adsorption coefficient of the test item was derived from its retention time and the calibration graph. Two series were injected. Each series included the calibration substances and the test item. The test item was injected in-between the series of the calibration substances. The adsorption coefficient of the test item was found to be: log Koc >4.20 (reference 5.4.1 -1).