1,4,7,10-tetraazacyclododecane

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: 92/69/EWG, C.2

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Analysis of the test medium, which were conducted during the experiment, confirming the conformity of the actual concentrations with the nominal concentrations.

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: M4, Vorschrift Dr. Elendt-Schneider

Test organisms (species):

Daphnia magna

Total exposure duration:

48 h

Hardness:

250 mg CaCO3/L

pH:

The pH of the test medium rose with the increasing concentration of test substance (Alkaline effect).Another test was conducted with HCl to keep HCl at 8.1

Nominal and measured concentrations:

Nominal concentrations of 10 to 100 mg / l.

Details on test conditions:

Test was conducted in duplicates with 10 Dapnias per vessel

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

86 mg/L

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

34 mg/L

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

100 mg/L

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Details on results:

%Concentration loss over test: 0 ... 5In the test series with adjusted pH (8.1) a slightly increased toxicity with respect to the immobilization of Daphnia Magna was observed with 10 mg/ L NOEC. In the test series with increasing pH, the EC50 value of 100 mg / l changed after 24 hours to 45 mg / L. After 48 hours, with a confidence interval of 95%. The EC50 values ​​were determined with 39-57 mg / l.In the test series with a constant pH (8.1), the EC50 value of 86 mg / l changed after 24 hours to 34 mg / l after 48 hours, with a confidence interval of 95%, the EC50 values ​​of 26-54 mg / l were determined ,

Conclusions:

The caustic effect of the test substance had no influence on the toxicity. EC50(24h): 86mg/LEC50(48h): 34 mg/LEC100(48h): 100mg/LNOEC(48h): 10mg/L

Executive summary:

The short term toxicity (48h) of test substance was performed in Daphnia Magna according to the 92/69/EWG, C.2 Guideline under GLP conditions.

The test was conducted twice unbuffered and buffered with HCl. The caustic effect of the test substance had no influence on the toxicity. The test substance short-term (24 and 48 h) effect parameters (mobility based) to Daphnia Magna were: EC50(24h): 86mg/L; EC50(48h): 34 mg/L; EC100(48h): 100mg/L; NOEC(48h): 10mg/L.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test organisms (species):

Daphnia magna

Total exposure duration:

48 h

Hardness:

254.22 mg CaCO3/ L

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

73.3 mg/L

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

25.1 mg/L

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

50 mg/L

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

12.5 mg/L

Details on results:

%Concentration loss over test: 1 ... 7

Toxic in the concentrations > 12.5 mg/L;

Analytical measurements by DOC analysis

Conclusions:

The test substance short-term (48 h) toxicity parameters in Daphnia Magna were:EC50(24h): 73.3 mg/LEC50(48h): 25.1 mg/LEC100(48h):50 mg/LNOEC(48h): 12.5 mg/L

Executive summary:

The short term toxicity (48h) of test substance was performed in Daphnia Magna according to the OECD 202 Guideline under GLP conditions.

The test substance short-term (24 and 48 h) effect parameters (mobility based) in Daphnia Magna were: EC50(24h): 73.3 mg/L; EC50(48h): 25.1 mg/L; EC100(48h):50 mg/L; NOEC(48h): 12.5 mg/L.

Description of key information

Short-term toxicity to aquatic invertebrates. Key study (001).Test method according to OECD 202 Guideline. GLP study.The short term toxicity (48h) of test substance was performed in Daphnia Magna.

EC50(48h): 25.1 mg/L.

Short-term toxicity to aquatic invertebrates. Key study (002). Test method according to 92/69/EWG, C.2 Guideline, comparable to guideline with acceptable restrictions. GLP study.The short term toxicity (48h) of test substance was performed in Daphnia Magna.The test was conducted twice unbuffered and buffered with HCl. The caustic effect of the test substance had no influence on the toxicity.

EC50(48h): 34 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

25.1 mg/L

Additional information

Justification for selection of short-term toxicity to aquatic invertebrates endpoint

Study 001 was selected because it is following the most recent guideline (OECD 202).