Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 951-169-0
CAS number: -
The test item Pentyl nitrite was assayed for the in
vitro skin corrosion in human epidermal model EpiDermTM.
The test was performed according toOECD Test Guideline 431,In Vitro Skin
Corrosion: Human Skin Model Test, Adopted: July 29th, 2016.
Complementary experiments were performed as a part of another
study:Study No. 293/18/5AI:Pentyl Nitrite,In vitro Eye
IrritationTest (EpiOcularTMmodel); VUOS-CETA Report
No.18-436, 2018,both with negative result. Colour of the test item does
not interfere with the endpoint and the test item has no direct
Therefore, results of MTT test did not need to be corrected.
In the MTT test, the test item (50 μL)wasplaced atop
the tissue.Length of exposition was 3 and60 minutes. Nine tissues were
used for the experiment in each time: three per test item (C1), three
for the positive control (PC) and three for the negative control (NC).
After rinsing, tissues were incubated with MTT for three hours and
then extracted with isopropyl alcohol for two hours at room temperature
with shaking.OD570of isopropyl alcohol extracts was measured
on a spectrophotometer. Relative cell viability was calculated for each
tissue as % of the mean viability of the negative control tissues.
Under the above-described experimental design, the average
viability of tissues treated with the test item Pentyl nitrite was
86.8 % of the negative control average value after 3 minutes
treatment and 1.8 % after 60 minutes of treatment.
The test item is considered to be corrosive to skin:
ii) if the viability after 3 minutes exposure is greater than or
equal to 50 % and the viability after 1 hour exposure is less than 15 %.
In the experiment arrangement described above, the test item Pentyl
nitrite was corrosive in theEpiDermTMmodel.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again