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EC number: 842-798-0 | CAS number: 444996-79-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study test phase 2013-08-13 to 2013-08-17.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Meets the criteria for classification as reliable without restriction according to Klimisch et al (1997).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- lithium 3-((3,4-dicyanophenyl)thio)propane-1-sulfonate
- EC Number:
- 700-787-7
- Cas Number:
- 769953-18-6
- Molecular formula:
- C11H9N2O3S2Li
- IUPAC Name:
- lithium 3-((3,4-dicyanophenyl)thio)propane-1-sulfonate
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Samples for analysis were taken at 0h from the excess test solutions and at 72h from a single test replicate solution of each concentration (containing alga). Samples containing algae were analysed post centrifugation.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- A nominal 120 mg/L primary stock solution of test material was prepared by dissolving 0.240 g in 2000 mL of culture medium. This stock solution was stirred for 10 minutes using magnetic follower. The stock solution was observed to be clear and colourless.
One litre of each test solution was prepared by the addition of aliquots of primary stock solution to sterile culture medium to final volumes of 1000 mL. The resultant solutions were clear and colourless. The control consisted of culture medium only. The appropriate test solution (100 mL volume) was dispensed to each test and blank vessel.
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test species was the unicellular green alga P. subcapitata blue green alga strain CCAP 278/4 from laboratory cultures maintained under axenic conditions. A 4 day old culture of the alga in the exponential growth phase was used as inoculum for the test. The culture was grown in the medium, and under the environmental conditions, described for the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22 +/-2 C
- pH:
- At the start of the test the pH ranged from 7.5 to 7.7 and at the end of the test the range was from 7.3 to 7.4. The maximum pH shift for the control replicates was 0.2.
- Nominal and measured concentrations:
- The limit of quantification in this study was 0.010 mg/l. The overall mean measured concentration ranged from 99 to 107% of the nominal values confirming that the test material was in solution. The nominal concentrations of test material were used in the calculating and reporting of the results.
- Details on test conditions:
- TEST SYSTEM
The test vessels were glass conical flasks of 250 mL nominal capacity closed with foam bungs. Each flask contained 100 mL of test solution. The cultures were incubated at 22 +/- 2°C (the nominal test temperature), under continuous "cool-white" illumination of approximately 6000 lux, with nominal orbital shaking at 160 rpm.
- Initial cells density: 5370 x104 cells/ml
- Control end cells density: 638000
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates):6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: no
- Intervals of water quality measurement: The pH of the control and test solutions was measured at the start of the test with a calibrated pH meter, using the excess remaining after filling the test vessels. At the end of the test the pH of one replicate vessel (containing algae) from each test concentration was determined. The temperature of the incubator was continuously monitored using an electronic recording system with hourly recording of values. The light intensity was measured once during the study, in each of four representative positions, using a photometer reading in lux (cosine).
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 'cool white' illumination of approx. 6000 lux.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: The algal cell densities of the inoculum and test cultures were determined by electronic particle counting, using a Coulter counter and counting at a lower threshold equivalent spherical diameter of approximately 2.3 µm. After 24, 48 and 72 hours, samples were removed from each test and blank vessel. The appropriate blank particle count was subtracted from that of the test culture to obtain the cell density.
TEST CONCENTRATIONS
- Test concentrations: 3.75, 7.5, 15, 30, 60 and 120 mg/l of test material - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 66 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL 53-75 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 45 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL 5.8-58 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 60 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 97 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL 87-106 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 60 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control : yes
- Observation of abnormalities: The microscopic observations made at test end showed that for the control and all test concentrations, the cells appeared normal.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72h EC50 was determined to be >120 mg/L, using biomass as a basis for effect. The 72h EC50, using growth rate as a basis for effect, was determined to be >120 mg/L.
- Executive summary:
Introduction
This study was undertaken to assess the toxicity of the test material to the green alga P.subcapitata. The test method was designed to be compatible with the following guidelines:
- OECD Guidelines for Testing of Chemicals. Test Guideline 201, Freshwater Alga and Cyanobacteria, Growth Inhibition Test. Adopted 23 March 2006.
- Official Journal of the European Communities, L383 A, Part C.3, Algal Inhibition test. 29 December 1992.
Results & Conclusions
The 72h EC50 was determined to be >120 mg/L, using biomass as a basis for effect. The 72h EC50, using growth rate as a basis for effect, was determined to be >120 mg/L.
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