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EC number: 700-261-7 | CAS number: 4427-96-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From October 28 to November 02, 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch number: 10121040902
Purity: 99.92% - Analytical monitoring:
- yes
- Details on sampling:
- During the test, the concentrations of the test chemical were analyzed in 0-hour and 48-hour fresh solution and 48-hour and 96-hour old solution.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1.1026 g test item were weighed and dissolved in 11 L of test water and then stirred for 24 hours to ensure maximum dissolution in test medium to get the stock solution of 100 mg/L. The stock solution was used for exposure and diluted to other test solutions. 0.50, 0.90, 1.60 and 2.80 L of stock solution of 100 mg/L were diluted to 5 L with test water to obtain the test solution of 10, 18, 32 and 56 mg/L. - Test organisms (species):
- other: Rare Minnow (Gobiocypris rarus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rare Minnow (Gobiocypris rarus)
- Source: The fish were reproduced in this facility. Their parental generation was introduced from CTI Chemical Safety Evaluation Research Center (SUZHOU) CO., LTD.
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 1-2 cm
ACCLIMATION
- Acclimation conditions (same as test or not): same as test
- Type and amount of food during acclimation:
- Feeding frequency during acclimation: Twice on weekday and one time on weekend until 24 hours before the test was started
- Health during acclimation (any mortality observed): no
FEEDING DURING TEST (as applicable)
- Food type: no
- Frequency: Fish were not fed during the period of the test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 40 to 250 mg/L (as CaCO3)
- Test temperature:
- 23±2 oC
- pH:
- 6.0 to 8.5
- Dissolved oxygen:
- Not less than 60% of air saturation value (ASV) during the test period
- Nominal and measured concentrations:
- Nominal concentrations: 10, 18, 32, 56 and 100 mg/L;
Measured concentrations: 3.36, 6.38, 9.68, 20.0 and 33.4 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass bottles, diameter: 16 cm, height: 26 cm
- Type (delete if not applicable): open / closed
- Aeration: Before usage, the water was aerated for more than 24 hours to attain dissolved oxygen saturation.
- Renewal rate of test solution (frequency/flow rate): 48 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): one
- No. of vessels per control (replicates): one
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: 0.0509 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the water treated with Aquapro Pure Water System
- Total organic carbon: < 2 mg/L
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hours light: 8 hours darkness
- Light intensity: 698 - 712 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Biological observation:
Observation intervals: at 2 ± 0.5 h, 5 ± 1 h and 24 ± 2 h after the start of the exposure (day 0-1). On days 2-4 of the test, all vessels with living fish should be inspected twice per day (preferably early morning and late afternoon to best cover the 24-hour periods).
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: During the period of 96 hours, no death or any visible toxic signs were observed in control group and test group of 10 mg/L. At the test group of 100 mg/L, no visible toxic sign was observed and all fish died during the test. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 30.66 mg/L
- 95% CI:
- 21.04 - 44.69
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- During the 96-hour exposure period, no death and visible abnormalities were observed in the control group and test groups of 10, 18 and 32 mg/L. One died fish was observed in the test group of 56 mg/L and four died fish were observed in the test group of 100 mg/L.
According to the results and based on the measured concentrations of test item, the 24, 48 hours LC50 of VEC to Rare Minnow (Gobiocypris rarus) was greater than 33.40 mg/L, and 72 and 96 hours LC50 of VEC to Rare Minnow (Gobiocypris rarus) was 30.66 mg/L, with 95% confidence limit of 21.04 – 44.69 mg/L. - Results with reference substance (positive control):
- 24-hour LC50: 299.86 mg/L
95% confidence limits: 241.82 mg/L ~ 371.83 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 hours LC50 of VEC to Rare Minnow (Gobiocypris rarus) was 30.66 mg/L.
- Executive summary:
A fish acute toxicity study of VEC was conducted with Rare Minnow (Gobiocypris rarus) over a 96-hour exposure period, according to OECD Guideline 203 under GLP.
According to the results and based on the measured concentrations of test item, the 24, 48 hours LC50 of VEC to Rare Minnow (Gobiocypris rarus) was greater than 33.40 mg/L, and 72 and 96 hours LC50 of VEC to Rare Minnow (Gobiocypris rarus) was 30.66 mg/L, with 95% confidence limit of 21.04 – 44.69 mg/L.
Reference
Description of key information
The 96 hours LC50 of VEC to Rare Minnow (Gobiocypris rarus) was 30.66 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 30.66 mg/L
Additional information
A fish acute toxicity study of VEC was conducted with Rare Minnow (Gobiocypris rarus) over a 96-hour exposure period, according to OECD Guideline 203 under GLP.
According to the results and based on the measured concentrations of test item, the 24, 48 hours LC50 of VEC to Rare Minnow (Gobiocypris rarus) was greater than 33.40 mg/L, and 72 and 96 hours LC50 of VEC to Rare Minnow (Gobiocypris rarus) was 30.66 mg/L, with 95% confidence limit of 21.04 – 44.69 mg/L.
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