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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Justification for type of information:
Please refer to the Category Approach Justification provided in Section 13
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
20 - 24 JAN 1997 and 03 - 07 FEB 1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes
Specific details on test material used for the study:
Trade Name: Marlowet BL
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: common carp (Cyprinus carpio)
- Source: Bio International B.V., Someren, NL
- Length at study initiation (length definition, mean, range and SD): 3 - 4 cm
- Feeding during test: no feeding


ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): 200 L dechlorinated drinking water in 300 L glass aquaria at 20°C ± 2°C, flow-through system
- Type and amount of food: Truovit, 1% of body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): 7 days prior test start: <= 5%
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
1st test: 12.5 ° dH
2nd test: 12 ° dH
Test temperature:
1st test: mean: 20 °C (19.4 - 20.7 °C)
2nd test: mean: 21 °C (20.5 - 20.9 °C)
pH:
1st test: 8.0 - 8.2
2nd test: 8.0 - 8.3
Dissolved oxygen:
1st test: 93% - 97%
2nd test: 72% - 97%
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 20 L aquaria filled with 10 L test medium
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.67 g

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water Gelsenwasser AG

OTHER TEST CONDITIONS
- Photoperiod: 16 light / 8 h darkness
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 1.0 - 1.7
Sublethal observations / clinical signs:

Concentration
[mg/L]

Test period (h)

24 h

48 h

72 h

96 h

Mortality [%]

Mortality [%]

Mortality [%]

Mortality [%]

1st test

Control

0

0

0

0

1.0

0

0

20

20

1.7

10

100

100

100

3.0

100

100

100

100

5.0

100

100

100

100

9.0

100

100

100

100

2nd test

Control

0

0

0

0

0.3

0

0

0

0

0.6

0

0

0

0

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
07 - 11 NOV 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes
Specific details on test material used for the study:
Trade name: Marlipal 24/20
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1 g of test substance/L was diluted in demineralized water and stirred over 18 h and filtered subsequently
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebrafish
- Source: West Aquarium, Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 3 cm +/- 0.5 cm
- Feeding during test: no feeding


ACCLIMATION
- Acclimation period: 14 days quarantine after treatment with malachit-green
- Acclimation conditions (same as test or not): dechlorinated drinking water in 200 L stone ware aquaria or 300 L glas aquaria, 20 °C +/- 2 °C, flow-
through conditions
- Type and amount of food: TetraMin approx. 1% of body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): animals were healthy at test start, mortality <= 5% prior test start

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
10.8 ° dH (2nd test), 10.5 ° dH (3rd test)
Test temperature:
20 °C
pH:
7.8 - 8.3 (2nd test)
7.8 - 8.4 (3rd test)
Dissolved oxygen:
80 - 101% (2nd test)
98 - 106% (3rd test)
Nominal and measured concentrations:
nominal: 0.12, 0.25, 0.50, 1.0, 2.0 mg/L (2nd test) and 3.6 and 6.0 mg /L (3rd test)
mean measured (geometric mean): 0.12, 0.12, 0.13, 0.46, 0.66 mg/L (2nd test) and 2.4 and 4.4 mg/L (3rd test)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 20 L test vessels filled with 10 L test water
- Aeration: continuous
- Renewal rate of test solution (frequency/flow rate): semi-static test - daily renewal of test water
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.25 g/fish (2. test), 0.20 g/fish (3. test)


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water (Gelsenwasser AG)
- Intervals of water quality measurement: daily


OTHER TEST CONDITIONS
- Photoperiod: 16 light / 8 h darkness

Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.2 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
0.66 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Sublethal observations / clinical signs:

Mean measured concentration
[mg/L]

Test period (h)

24 h

48 h

72 h

96 h

Mortality [%]

Mortality [%]

Mortality [%]

Mortality [%]

2nd test

Control

0

0

0

0

0.12

0

0

0

0

0.12

0

0

0

0

0.13

0

0

0

0

0.46

0

0

0

0

0.56

0

0

0

0

3rd test

Control

0

0

0

0

2.4

100

100

100

100

4.4

100

100

100

100

Validity criteria fulfilled:
yes
Conclusions:
The 96-hour LC50 of Alcohols, C12-14, ethoxylated (2 EO) to Brachydanio rerio was investigated in accordance to EU Guideline 92/69 EWG Part C 1. The LC50 was determined to be 1.2 mg/l.

Description of key information

LC50 (96h) = 1.2 mg/L for fish (Cyprinus carpio and Danio rerio)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
1.2 mg/L

Additional information

Since no data for decan-1-ol, ethoxylated, 1-2.5EO (CAS 26183-52-8), are available the acute toxicity on fish is assessed on the basis of data from studies with similar alcohol ethoxylates (C12, 4 EO; CAS 9002-92-0, and C12-14, 2EO; CAS 68439-50-9). As aquatic toxicity increases with an increase in the length of the hydrocarbon chain the resulting LC50 values can be considered as worst case.

The first study was carried out according to GLP and EU Method C.1 (Acute Toxicity for Fish) with analytical verification of the test concentrations (Sasol, 1997). Carp (Cyprinus carpio) was exposed for 96 h under semi-static conditions. Based on nominal concentrations, an LC50 (96h) of 1.2 mg/L is reported.

The second study (Sasol, 1995) was carried out according to GLP and EU Method C.1 (Acute Toxicity for Fish) as well. And analytical verification of the test concentrations was performed. Zebrafish (Danio rerio) was exposed for 96 h under semi-static conditions. Based on measured concentrations (geo. mean), an LC50 (96h) of 1.2 mg/L was derived.