Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 950-480-9 | CAS number: -
Oxygen consumption by activated sludge in presence of different concentrations of Algea Fert Solid K+
Test item concentration
Oxygen concentration (mg/L)
n.d.: not determined as the values dropped below the 2.5 mg O2/L value.
Oxygen consumption by activated sludge in presence of different concentrations of 3,5-dichlorophenol
Reference item concentration
Respiratory rates for the test concentrations and for the controls and active sludge respiration inhibition caused by test item after 3-h exposure
* negative values indicate that a respiration greater than the mean respiration of two controls occurred, as provided by biological variability
Respiratory rates for the reference item concentrations and active sludge respiration inhibition in comparison to controls after 3-h exposure
The toxicity of the test item Algea Fert Solid K+ to activated sludge was assessed in a respiration inhibition study according to:
- EU Commission Directive EC 440/2008, C.11.
- OECD Guideline No. 209, “Activated Sludge, Respiration Inhibition Test”, 1984.
For this purpose, aerobic activated sludge collected from the recirculation tank of a municipal wastewater treatment plant was incubated for 3-hours with different concentrations of the test item under defined conditions.
The test item concentrations were 10.0, 17.8, 31.6, 56.2 and 100.0 mg/L. In addition to the five test item concentrations, two untreated controls were incubated under the same conditions.
In order to check the normal sensitivity of the batch of activate sludge, 3,5-dichlorophenol was used as a reference item. For this purpose three nominal concentrations of 3,5-dichlorophenol were tested, 5.0, 15.0 and 50.0 mg/L respectively.
The EC50of 3,5-dichlorophenol was found to be 11.79 mg/L, thus complying with the validity range indicated by the referenced guidelines (EC50: 5-30 mg/L).
The respiratory rate expressed as oxygen consumption was measured after three hours of exposure using a portable oximeter.
The inhibitory effect of the test item is expressed as a percent of the mean respiratory rate of two controls. The results are related to the nominal concentrations of the test item.
The respiration rate of the two controls was found to differ by 10.8%, thus complying with the validity criteria indicated by the mentioned guidelines (maximum difference between two replicates of control = 15%).
Based on the results, the EC20, EC50and the EC80for the test item were assessed to be greater than the highest tested concentration, i.e. greater than 100 mg/L.
At the start of the test the pH of test water was 7.43. The pH values and temperature of test solutions were measured in each test flask during the oxygen determination: the pH was in the range 7.80 – 8.00, while the temperature was found to be in the range 19.7 – 20.3°C, i.e. within the specified range (20.0± 2 °C).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again